Developing an alginate/chitosan hybrid fiber scaffold for annulus fibrosus cells

A fibrous scaffold made of alginate or alginate/chitosan was fabricated for annulus fibrosus (AF) cell culture using a wet‐spinning and lyophilization technique. The scaffolds were evaluated using several in vitro tests. Scanning electron microscopy showed the scaffold fibers generally aligned in on...

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Bibliographic Details
Published in:Journal of biomedical materials research. Part A 2007-09, Vol.82A (3), p.701-710
Main Authors: Shao, Xinxin, Hunter, Christopher J.
Format: Article
Language:English
Subjects:
3T3 Cells
alginate
Alginates - pharmacology
Alginates - therapeutic use
Animals
annulus fibrosus
Cell Culture Techniques
Cell Survival - drug effects
chitosan
Chitosan - pharmacology
Chitosan - therapeutic use
Dogs
Extracellular Matrix
Fibroblasts - cytology
Glucuronic Acid - pharmacology
Glucuronic Acid - therapeutic use
Hexuronic Acids - pharmacology
Hexuronic Acids - therapeutic use
intervertebral disc
Intervertebral Disc - cytology
Mice
Tissue Engineering - methods
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Summary:A fibrous scaffold made of alginate or alginate/chitosan was fabricated for annulus fibrosus (AF) cell culture using a wet‐spinning and lyophilization technique. The scaffolds were evaluated using several in vitro tests. Scanning electron microscopy showed the scaffold fibers generally aligned in one direction with individual fiber diameters varying between 40–100 μm. The alginate/chitosan hybrid scaffold exhibited a slower degradation rate, while both scaffold types did not display any cyto‐toxicity to 3T3 fibroblasts and could maintain canine AF cell growth. The AF cells retained their spherical shape within the fibrous scaffold at the beginning of the culture period and formed into cell clusters at later times. Specific extracellular matrix molecules, including collagen I, collagen II, and aggrecan, could be detected in the AF cell clusters. These results demonstrate the feasibility of using this hybrid alginate/chitosan scaffold for AF cell culture, and the potential application for intervertebral disc tissue engineering. © 2007 Wiley Periodicals, Inc. J Biomed Mater Res 2007
ISSN:1549-3296
1552-4965
DOI:10.1002/jbm.a.31030