Development of an ELISA for the detection of fowl adenovirus serotype −4 utilizing fiber protein

Hydropericardium syndrome (HPS), caused by the Fowl adenovirus 4 (FAdV-4) has led to significant financial losses for the poultry industry globally, including Pakistan over the past few years. Conventional serological methods are time consuming, laborious and less sensitive therefore, a rapid and se...

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Bibliographic Details
Published in:Biologicals 2024-02, Vol.85, p.101752-101752, Article 101752
Main Authors: Ather, Faiza, Zia, Muhammad Ashir, Habib, Mudasser, Shah, Muhammad Salah-ud-din
Format: Article
Language:English
Subjects:
Adenoviridae Infections - diagnosis
Adenoviridae Infections - veterinary
Animals
Antibodies, Viral
Aviadenovirus - genetics
Chickens
E. coli
ELISA
Enzyme-Linked Immunosorbent Assay - methods
FAdV-4
Fiber proteins
Poultry Diseases
Serogroup
Specificity
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Summary:Hydropericardium syndrome (HPS), caused by the Fowl adenovirus 4 (FAdV-4) has led to significant financial losses for the poultry industry globally, including Pakistan over the past few years. Conventional serological methods are time consuming, laborious and less sensitive therefore, a rapid and sensitive ELISA kit is required for the reliable detection of FAdV-4 infection. In the current research, fiber proteins (1 &2) of FAdV-4 were successfully expressed in Escherichia coli and purified using metal affinity chromatography. Using these proteins as antigens, an indirect ELISA for detecting FAdV-4 infection was developed. The developed ELISA showed superior performances upon comparison with Serum neutralization test (SNT). This ELISA also showed reliable detection of FAdV specific antibodies in experimentally infected and vaccinated chickens. This assay produced good correlation on the samples collected from the field with SNT and found essential for large scale serology of the FAdV. No cross reactivity was observed in the ELISA following the testing of the serum samples of different other avian pathogens which showed that this ELISA is specific in detecting the FAdV infection. In conclusion, the developed Fiber protein ELISA is highly sensitive and specific in the detecting the FAdV infection and can be utilized for large scale sero-epidemiology of the disease.
ISSN:1045-1056
1095-8320
DOI:10.1016/j.biologicals.2024.101752