Development and Validation of UPLC Tandem Mass Spectrometry Assay for Ceftibuten and Sulbactam in Human Plasma

A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of wa...

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Bibliographic Details
Published in:Journal of chromatographic science 2024-03
Main Authors: Valero-Rivera, Karla Paulina, Magaña-Aquino, Martin, Del Carmen Romero-Méndez, María, Morales-Barragán, Miriam Nayeli, Sagahón-Azúa, Julia, Medellín-Garibay, Susanna Edith, Del Carmen Milán-Segovia, Rosa, Martínez-Gutiérrez, Fidel, Romano-Moreno, Silvia
Format: Article
Language:English
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Summary:A sensitive and rapid ultra-performance liquid chromatography coupled with -tandem mass spectrometry (UPLC-MS/MS) method was developed and validated to determine ceftibuten (CTB) and sulbactam (SUL) in human plasma. An ACQUITY UPLC HSS T3 C18 (2.1 × 100 mm), 1.8 μm column with gradient elution of water (0.1% formic acid) and acetonitrile was used for separation at a flow rate of 0.2 mL/min. This method involves a simple sample preparation with acetonitrile. The calibration curves of CTB and SUL in plasma showed good linearity over the concentration range of 0.50-25 μg/mL and with a correlation coefficient (r2) >0.99. This method was validated in terms of selectivity, linearity, precision, accuracy and stability. High precision was obtained with coefficients of variation
ISSN:0021-9665
1945-239X
DOI:10.1093/chromsci/bmae010