Quinone Methide Based Self‐Immobilizing Molecular Fluorescent Probes for In Situ Imaging of Enzymes

Enzymes play important roles not only in normal physiological processes but in the development of many diseases. In situ imaging of enzymes with high‐resolution in living systems would helpful for clinical diagnosis and treatment. However, many molecular fluorescent probes suffer from the drawback o...

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Bibliographic Details
Published in:Chemistry, an Asian journal an Asian journal, 2024-05, Vol.19 (10), p.e202400189-n/a
Main Authors: Miao, Yeru, Yu, Zhen‐Qing, Xu, Shuai, Yan, Mei
Format: Article
Language:English
Subjects:
Enzymes
Enzymes - chemistry
Enzymes - metabolism
Fluorescent Dyes - chemical synthesis
Fluorescent Dyes - chemistry
Fluorescent indicators
Humans
Image resolution
Immobilization
Indolequinones - chemistry
in situ imaging
Medical imaging
Molecular Structure
Optical Imaging
quinone methide
Quinones
self-immobilizing probe
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Summary:Enzymes play important roles not only in normal physiological processes but in the development of many diseases. In situ imaging of enzymes with high‐resolution in living systems would helpful for clinical diagnosis and treatment. However, many molecular fluorescent probes suffer from the drawback of diffusing away from the reaction site of enzymes even out of the cells, losing the in situ information and resulting in poor imaging resolution. Quinone methide (QM) based self‐immobilizing probes allow the fluorescent signal to be immobilized near the target for an extended period without deactivating the target enzymes, ensuring that it will provide amplified signals and in situ information of the target with high resolution. In this review, we summarized the recent progress of QM‐based self‐immobilizing probes including their design strategies, working mechanisms, classifications and applications in in situ enzyme imaging. This review calls for the development of more activatable QM‐based probe with the advantages of high stability in the absence of the target but very high labeling efficiency after activation. Quinone methide (QM) based self‐immobilizing probes can provide the amplified signals and in situ information of the target without deactivating the target enzymes. This review summarized the recent progress of QM‐based self‐immobilizing probes including their design strategies, working mechanisms, classifications and applications in in situ enzyme imaging, which is expected to inform the interested researchers.
ISSN:1861-4728
1861-471X
DOI:10.1002/asia.202400189