Sperm characteristics of cryopreserved Prochilodus lineatus semen after adding cholesterol-loaded cyclodextrin

The experiment evaluated the effect of adding cholesterol-loaded cyclodextrin (CLC) to Prochilodus lineatus fish (Curimata) semen on post-thaw sperm quality. Twelve adult fish were used for sperm collection after induced spermiation with carp pituitary gland. The semen was diluted and treated with C...

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Bibliographic Details
Published in:Cryobiology 2024-06, Vol.115, p.104888-104888, Article 104888
Main Authors: Antunes, K.V., Santos, J.J.S., Carvalho, I.C.S., Moreira, E.M.S., Moreira, G.R., Murgas, L.D.S., Moraes, E.A.
Format: Article
Language:English
Subjects:
Fish
Membrane integrity
Morphology
Motility
Sperm longevity
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Summary:The experiment evaluated the effect of adding cholesterol-loaded cyclodextrin (CLC) to Prochilodus lineatus fish (Curimata) semen on post-thaw sperm quality. Twelve adult fish were used for sperm collection after induced spermiation with carp pituitary gland. The semen was diluted and treated with CLC in concentrations of 0 (control), 0.5, 1.0, 2.0, 3.0, and 4.0 mg for 120 × 106 spermatozoa/ml, loaded in 0.5 ml straws, packaged and placed in dry vapor vessel cylinders for 24 h before being submerged in liquid nitrogen for storage. The samples were thawed in a water bath at 60 °C for 8 s, and the sperm parameters evaluated were motility, activation duration, longevity, plasma membrane integrity, and morphology. Data were tested for normal distribution and ANOVA, followed by Friedman test (P 
ISSN:0011-2240
1090-2392
DOI:10.1016/j.cryobiol.2024.104888