Comparative identification of WRKY transcription factors and transcriptional response to Ralstonia solanacearum in tomato

•Tomato WRKY transcription factors (TFs) were comprehensively identified and characterized.•Several positive or negative regulators in response to tomato bacterial wilt were transcriptionally evaluated.•Solyc03g095770.3 interacting with Solyc09g014990.4 may play an important role in response to toma...

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Published in:Gene 2024-06, Vol.912, p.148384-148384, Article 148384
Main Authors: Shui, Deju, Sun, Ji, Xiong, Zili, Zhang, Shengmei, Shi, Jianlei
Format: Article
Language:English
Subjects:
Gene expression
Genome-wide investigation
Homologs
Tomato bacterial wilt
WRKY TFs
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Summary:•Tomato WRKY transcription factors (TFs) were comprehensively identified and characterized.•Several positive or negative regulators in response to tomato bacterial wilt were transcriptionally evaluated.•Solyc03g095770.3 interacting with Solyc09g014990.4 may play an important role in response to tomato bacterial wilt. In order to study the responses of tomato (Solanum lycopersicum) WRKY TFs to bacterial wilt caused by Ralstonia solanacearum, the most up-to-date genomes and transcriptional profiles were used to identify WRKY TFs in control and infected inbred lines. In total, 85 tomato WRKY TFs were identified and categorized into groups I, IIa + b, IIc, IId + e, and III. These WRKYs, especially those from group IIe, were mainly distributed at chromosome ends and in clusters. More than 45 % and 70 % of tomato WRKYs exhibited intraspecific and interspecific synteny, respectively. Nearly 60 % of tomato WRKYs (mainly in groups I and IIc) formed 73 pairs of orthologs with WRKYs in Arabidopsis and pepper, with Ka/Ks less than 1. Sixteen tomato WRKYs (mainly in groups IIa + b and IIc) responded strongly to biotic stress, and 12 differentially expressed WRKYs (mainly in groups III and IIb) were identified. RT-qPCR revealed that tomato WRKYs could respond to bacterial wilt through positive (predominant) or negative regulation. In particular, the interaction between Solyc03g095770.3 (group III) and Solyc09g014990.4 (group I) may play an important role. In brief, WRKY TFs were comprehensively identified in tomato and several bacterial wilt responsive genes were screened.
ISSN:0378-1119
1879-0038
DOI:10.1016/j.gene.2024.148384