Pro-inflammatory enzyme inhibition of lipoxygenases by flavonoid rich extract from Artemisia vulgaris

[Display omitted] •Ionic-liquid based ultrasonic extraction of flavonoids from A. vulgaris was optimized.•Extraction time was more significant than solid-to-liquid ratio for high flavonoids.•Michaelis-Menten kinetics was used to study the inhibition mode of flavonoids.•Inhibition of flavonoid extrac...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2024-04, Vol.1237, p.124072-124072, Article 124072
Main Authors: Lim, Jin Ru, Chua, Lee Suan, Mustaffa, Azizul Azri
Format: Article
Language:English
Subjects:
Antioxidants - pharmacology
Artemisia
Artemisia vulgaris
Flavonoids
Flavonoids - pharmacology
Ionic liquids
Lipoxygenases
LOX inhibition activity
Plant Extracts - pharmacology
Ultrasound assisted extraction
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Summary:[Display omitted] •Ionic-liquid based ultrasonic extraction of flavonoids from A. vulgaris was optimized.•Extraction time was more significant than solid-to-liquid ratio for high flavonoids.•Michaelis-Menten kinetics was used to study the inhibition mode of flavonoids.•Inhibition of flavonoid extracts against lipoxygenases showed a mixed inhibition mode.•A. vulgaris is a potent herbal plant for anti-inflammatory properties. The peroxyl radicals generated by the activity of lipoxygenases (LOX) are mediators to trigger inflammatory diseases. Therefore, it is important to investigate potent LOX inhibitor for modulating the occurrence and resolving inflammatory processes. Artemisa vulgaris, is a herbal plant that is known for flavonoids, potentially inhibiting lipid peroxidation and scavenging radicals. The objectives of the present study were to obtain flavonoids rich extract from A. vulgaris, and determine the inhibitory mode of the extract against LOX. The flavonoids rich extract was optimized in an ultrasound assisted extraction using ionic liquids as extraction solvent. The results found that the optimum conditions; ratio of solid-to-liquid (1:10) and 30 min of extraction time could produce the high yield (10.14 %) and flavonoid content (5.30 mg QE/g). The LOX activity was demonstrated to follow a mixed mode of inhibition in the presence of the flavonoid rich extract as an inhibitor. The Michaelis-Menten constant (Km) was increased from 0.283 µM to 0.435 µM, whereas the maximum velocity was reduced from 0.22 µM/min to 0.058 µM/min in the inhibition. The flavonoids rich extract is likely to be a natural potent non-competitive inhibitor which may bind to free LOX or substrate-bound LOX.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2024.124072