Binding Dynamics of a Stapled Peptide Targeting the Transcription Factor NF−Y

Transcription factors (TFs) play a central role in gene regulation, and their malfunction can result in a plethora of severe diseases. TFs are therefore interesting therapeutic targets, but their involvement in protein–protein interaction networks and the frequent lack of well–defined binding pocket...

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Bibliographic Details
Published in:Chembiochem : a European journal of chemical biology 2024-05, Vol.25 (9), p.e202400020-n/a
Main Authors: Durukan, Canan, Arbore, Federica, Klintrot, Rasmus, Bigiotti, Carlo, Ilie, Ioana M., Vreede, Jocelyne, Grossmann, Tom N., Hennig, Sven
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Binding
Binding Sites
biophysics
CCAAT-Binding Factor - chemistry
CCAAT-Binding Factor - metabolism
Crystallography, X-Ray
flexibility
Gene regulation
Humans
Molecular dynamics
Molecular Dynamics Simulation
Peptides
Peptides - chemistry
Peptides - metabolism
peptidomimetic
Protein Binding
protein crystallography
Protein interaction
Proteins
protein–protein interaction
Therapeutic targets
Transcription factors
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Summary:Transcription factors (TFs) play a central role in gene regulation, and their malfunction can result in a plethora of severe diseases. TFs are therefore interesting therapeutic targets, but their involvement in protein–protein interaction networks and the frequent lack of well–defined binding pockets render them challenging targets for classical small molecules. As an alternative, peptide–based scaffolds have proven useful, in particular with an α‐helical active conformation. Peptide–based strategies often require extensive structural optimization efforts, which could benefit from a more detailed understanding of the dynamics in inhibitor/protein interactions. In this study, we investigate how truncated stapled α‐helical peptides interact with the transcription factor Nuclear Factor‐Y (NF−Y). We identified a 13‐mer minimal binding core region, for which two crystal structures with an altered C‐terminal peptide conformation when bound to NF−Y were obtained. Subsequent molecular dynamics simulations confirmed that the C‐terminal part of the stapled peptide is indeed relatively flexible while still showing defined interactions with NF−Y. Our findings highlight the importance of flexibility in the bound state of peptides, which can contribute to overall binding affinity. A truncated version of a stapled peptide maintains moderate affinity for the for transcription factor complex NF−Y. Interestingly, the peptide's C‐terminal region shows considerable flexibility as confirmed by crystal structures and molecular dynamics simulations pointing, towards the importance of ligand flexibility in the bound state.
ISSN:1439-4227
1439-7633
DOI:10.1002/cbic.202400020