Investigating the Effects and Mechanisms of Cyclomorusin on Osteoclasts in a High Glucose Environment

Diabetes mellitus is an endocrine disease characterized by prolonged hyperglycemia. Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory pr...

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Bibliographic Details
Published in:Chemistry & biodiversity 2024-05, Vol.21 (5), p.e202301741-n/a
Main Authors: Guo, Dong‐Gui, Zhu, Jun, Wang, Hui‐Juan, Pan, Bo‐Wen
Format: Article
Language:English
Subjects:
Animals
Blood levels
Bone resorption
Bone Resorption - drug therapy
Bone Resorption - metabolism
Cell culture
Cell Differentiation - drug effects
Cells, Cultured
Cyclomorusin
Diabetes mellitus
Differentiation
Dose-Response Relationship, Drug
Endocrine disorders
Gene expression
Genes
Glucose - metabolism
Glucose - pharmacology
High glucose concentrations
Hyperglycemia
Maturation
Mercury
Mice
mRNA
Ocean bottom seismometers
Osteoclasts
Osteoclasts - cytology
Osteoclasts - drug effects
Osteoclasts - metabolism
Osteoporosis
RANK Ligand - metabolism
RANKL/RANK/TRAF6 pathway
RAW 264.7 Cells
Receptor Activator of Nuclear Factor-kappa B - genetics
Receptor Activator of Nuclear Factor-kappa B - metabolism
Saccharides
TNF Receptor-Associated Factor 6 - metabolism
TRAF6 protein
Western blotting
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Summary:Diabetes mellitus is an endocrine disease characterized by prolonged hyperglycemia. Prolonged high blood sugar levels interfere with the differentiation and maturation process of OBs and OCs, leading to the onset of osteoporosis. However, OCs differentiation and maturation is a complex regulatory process. In this study, we used a co‐culture system of RAW264.7 and MC3T3‐E1 cells under HG concentration to explore the effect of CYM on OCs in a HG environment. The effects of CYM on the formation and function of OCs were observed using TRAP‐positive cell counts and bone resorption pits. Then, mRNA and protein expression levels of OCs‐related genes were detected by real‐time qPCR and western blotting. The results showed that CYM had an inhibitory effect on OCs differentiation and bone resorption, reduced mRNAs expression of OCs‐associated genes, and downregulated RANKL/RANK/TRAF6 pathway that mediates OCs differentiation. CYM could be a promising natural compound against diabetic osteoporosis.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202301741