A Microstructure Heat Exchanger for Quenching the Metabolism of Mammalian Cells
Metabolomics, the quantification of intracellular metabolites, is a powerful tool that has proven useful in microbial strain and fermentation process development. The crucial step is the rapid quenching of the cellular metabolism to preserve the intracellular situation as it is in the bioreactor unt...
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| Published in: | Chemical engineering & technology 2007-03, Vol.30 (3), p.322-328 |
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| Main Authors: | , , , , , , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Request full text |
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| Summary: | Metabolomics, the quantification of intracellular metabolites, is a powerful tool that has proven useful in microbial strain and fermentation process development. The crucial step is the rapid quenching of the cellular metabolism to preserve the intracellular situation as it is in the bioreactor until (MS‐based) analysis. For microbial systems and plants this can be achieved by using ice‐cold methanol (–40 °C) or liquid nitrogen, but for mammalian cells in suspension no reliable technique is yet available. Here, a metallic microstructure heat exchanger is presented which allows cooling of a CHO cell suspension from cultivation temperature (37 °C) to 0 °C in less than one second without any detectable cell damage or cell disruption even at very high cell densities up to 8 · 106 cells/mL.
Investigating the metabolome of a cell requires rapid quenching of the metabolism, quantitative extraction, and highly sensitive analysis of the metabolites. A metallic microstructured heat exchanger is introduced which allows fast cooling of mammalian cell suspensions without any detectable cell damage or cell disruption. |
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| ISSN: | 0930-7516 1521-4125 |