Peroxisome-based metabolic engineering for biomanufacturing and agriculture

Peroxisomes are favorable platforms for organelle engineering, featuring abundant endogenous metabolites, optimal membrane permeability, frequent contacts with other organelles, efficient protein targeting signals, and many known organelle proliferation factors.Yeast peroxisome engineering has produ...

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Published in:Trends in biotechnology (Regular ed.) 2024-09, Vol.42 (9), p.1161-1176
Main Authors: Song, Shuyan, Ye, Cuifang, Jin, Yijun, Dai, Huaxin, Hu, Jianping, Lian, Jiazhang, Pan, Ronghui
Format: Article
Language:English
Subjects:
Agriculture
Alcohol
Biosynthesis
Biotechnology
Carbon
Chloroplasts
Efficiency
Engineering
Enzymes
Host plants
Metabolic engineering
Metabolic pathways
Metabolism
Metabolites
Mitochondria
Natural products
Oxidation
peroxisome
plant
Proteins
subcellular compartmentalization
Sugarcane
synthetic biology
Yeast
Yeasts
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Summary:Peroxisomes are favorable platforms for organelle engineering, featuring abundant endogenous metabolites, optimal membrane permeability, frequent contacts with other organelles, efficient protein targeting signals, and many known organelle proliferation factors.Yeast peroxisome engineering has produced valuable biomolecules, such as biopolyesters, biofuels, terpenoids, alkaloids, antibiotics, and phytohormones.Plant peroxisome engineering has great potential in biomanufacturing and agriculture and adds values to improving environmental sustainability and food security.Approaches for improving the performance of heterologous pathways in peroxisomes include increasing peroxisomal abundance, coupling multiple subcellular compartments, maximizing precursor and cofactor supplies, uncovering the rate-limiting steps, and peroxisomal surface display of enzymes. Subcellular compartmentalization of metabolic pathways plays a crucial role in metabolic engineering. The peroxisome has emerged as a highly valuable and promising compartment for organelle engineering, particularly in the fields of biological manufacturing and agriculture. In this review, we summarize the remarkable achievements in peroxisome engineering in yeast, the industrially popular biomanufacturing chassis host, to produce various biocompounds. We also review progress in plant peroxisome engineering, a field that has already exhibited high potential in both biomanufacturing and agriculture. Moreover, we outline various experimentally validated strategies to improve the efficiency of engineered pathways in peroxisomes, as well as prospects of peroxisome engineering. Subcellular compartmentalization of metabolic pathways plays a crucial role in metabolic engineering. The peroxisome has emerged as a highly valuable and promising compartment for organelle engineering, particularly in the fields of biological manufacturing and agriculture. In this review, we summarize the remarkable achievements in peroxisome engineering in yeast, the industrially popular biomanufacturing chassis host, to produce various biocompounds. We also review progress in plant peroxisome engineering, a field that has already exhibited high potential in both biomanufacturing and agriculture. Moreover, we outline various experimentally validated strategies to improve the efficiency of engineered pathways in peroxisomes, as well as prospects of peroxisome engineering.
ISSN:0167-7799
1879-3096
1879-3096
DOI:10.1016/j.tibtech.2024.02.005