Aromatase (CYP19) gene as a biomarker for detection of naphthalene and phenanthrene in Colombo to Mirissa coastal water in Sri Lanka

Naphthalene (NAP) and phenanthrene (PHE) are prevalent Polycyclic Aromatic Hydrocarbons (PAHs) in the environment. High-Performance Liquid Chromatography (HPLC) analysis was performed on marine water samples (n = 57) collected from 19 locations. Molecular screening of the aromatase (CYP19) gene expr...

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Bibliographic Details
Published in:Marine pollution bulletin 2024-04, Vol.201, p.116187-116187, Article 116187
Main Authors: Mallikarachchi, K.E.P., Bandara, K.R.V., Weerasekera, M.M., Nugara, R.N., Undugoda, L.J.S., Manage, P.M.
Format: Article
Language:English
Subjects:
Aromatase - genetics
Biomarkers
CYP19 gene
Naphthalene
Naphthalenes - analysis
Phenanthrene
Phenanthrenes - analysis
Polycyclic aromatic hydrocarbons (PAHs)
Polycyclic Aromatic Hydrocarbons - analysis
Seawater
Sri Lanka
Water - analysis
Water Pollutants, Chemical - analysis
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Summary:Naphthalene (NAP) and phenanthrene (PHE) are prevalent Polycyclic Aromatic Hydrocarbons (PAHs) in the environment. High-Performance Liquid Chromatography (HPLC) analysis was performed on marine water samples (n = 57) collected from 19 locations. Molecular screening of the aromatase (CYP19) gene expression was examined using quantitative Reverse Transcriptase PCR (qRT-PCR). The findings of the study showed a significant range of naphthalene concentrations along the coastline, spanning from 1.70 to 15.05 mg/L, where phenanthrene concentrations varied from undetectable to a maximum of 5.36 mg/L. The relative expression of the CYP19 gene ranged from 0.5 to 13.9 in the sampling sites. The ANOVA analysis showed a significant positive correlation (p 
ISSN:0025-326X
1879-3363
DOI:10.1016/j.marpolbul.2024.116187