Accumulation of defense systems in phage-resistant strains of Pseudomonas aeruginosa

Prokaryotes encode multiple distinct anti-phage defense systems in their genomes. However, the impact of carrying a multitude of defense systems on phage resistance remains unclear, especially in a clinical context. Using a collection of antibiotic-resistant clinical strains of and a broad panel of...

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Bibliographic Details
Published in:	Science advances 2024-02, Vol.10 (8), p.eadj0341-eadj0341
Main Authors:	Costa, Ana Rita, van den Berg, Daan F, Esser, Jelger Q, Muralidharan, Aswin, van den Bossche, Halewijn, Bonilla, Boris Estrada, van der Steen, Baltus A, Haagsma, Anna C, Fluit, Ad C, Nobrega, Franklin L, Haas, Pieter-Jan, Brouns, Stan J J
Format:	Article
Language:	eng
Subjects:	Anti-Bacterial Agents Bacteriophages - genetics Humans Pseudomonas aeruginosa Pseudomonas Infections - microbiology Pseudomonas Phages - genetics
Online Access:	Get full text
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Summary:	Prokaryotes encode multiple distinct anti-phage defense systems in their genomes. However, the impact of carrying a multitude of defense systems on phage resistance remains unclear, especially in a clinical context. Using a collection of antibiotic-resistant clinical strains of and a broad panel of phages, we demonstrate that defense systems contribute substantially to defining phage host range and that overall phage resistance scales with the number of defense systems in the bacterial genome. We show that many individual defense systems target specific phage genera and that defense systems with complementary phage specificities co-occur in genomes likely to provide benefits in phage-diverse environments. Overall, we show that phage-resistant phenotypes of with at least 19 phage defense systems exist in the populations of clinical, antibiotic-resistant strains.
ISSN:	2375-2548