mRNA biomarkers sensitive and specific to micro-dose erythropoietin treatment at sea level and altitude

Recombinant human erythropoietin (rhEPO) is prohibited by the World Anti-Doping Agency. rhEPO abuse can be indirectly detected via the athlete biological passport (ABP). However, altitude exposure challenges interpretation of the ABP. This study investigated whether 5'-aminolevulinate synthase...

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Published in:Drug testing and analysis 2024-02
Main Authors: Loria, Francesco, Breenfeldt Andersen, Andreas, Bejder, Jacob, Bonne, Thomas, Grabherr, Silke, Kuuranne, Tiia, Leuenberger, Nicolas, Baastrup Nordsborg, Nikolai
Format: Article
Language:English
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Summary:Recombinant human erythropoietin (rhEPO) is prohibited by the World Anti-Doping Agency. rhEPO abuse can be indirectly detected via the athlete biological passport (ABP). However, altitude exposure challenges interpretation of the ABP. This study investigated whether 5'-aminolevulinate synthase 2 (ALAS2) and carbonic anhydrase 1 (CA1) in capillary dried blood spots (DBSs) are sensitive and specific markers of rhEPO treatment at altitude. ALAS2 and CA1 expression was monitored in DBS collected weekly before, during, and after a 3-week period at sea level or altitude. Participants were randomly assigned to receive 20 IU kg bw epoetin alpha (rhEPO) or placebo injections every second day for 3 weeks while staying at sea level (rhEPO, n = 25; placebo, n = 9) or altitude (rhEPO, n = 12; placebo, n = 27). ALAS2 and CA1 expression increased up to 300% and 200%, respectively, upon rhEPO treatment at sea-level and altitude (P-values
ISSN:1942-7603
1942-7611
DOI:10.1002/dta.3665