Genotoxic effects and mitosis aberrations of chromium (VI) on root cells of Vicia faba and its molecular docking analysis

Like other heavy metals, Cr (VI) is a powerful carcinogen and mutagen agent. Its toxic effects on plants are well considered. In order to elucidate its adverse effects, the present work aims to study the mitosis aberrations of Cr (VI) on the Vicia faba root-cells and its molecular docking analysis t...

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Published in:Plant physiology and biochemistry 2024-02, Vol.207, p.108361-108361, Article 108361
Main Authors: Bouhadi, Mohammed, Abchir, Oussama, Yamari, Imane, El Hamsas El Youbi, Amal, Azgaoui, Anas, Chtita, Samir, El Hajjouji, Houda, El Kouali, M'hammed, Talbi, Mohammed, Fougrach, Hassan
Format: Article
Language:English
Subjects:
Chromium - toxicity
Chromium VI
Chromosome Aberrations
Colchicine - pharmacology
DNA Damage
Fabaceae
Genotoxicity
Ligases
Micronuclei
Mitosis
Mitosis aberrations
Molecular Docking Simulation
Tubulin - genetics
Tubulin - pharmacology
Tyrosine
Vicia faba - genetics
Vicia faba L
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Summary:Like other heavy metals, Cr (VI) is a powerful carcinogen and mutagen agent. Its toxic effects on plants are well considered. In order to elucidate its adverse effects, the present work aims to study the mitosis aberrations of Cr (VI) on the Vicia faba root-cells and its molecular docking analysis to understand the genotoxicity mechanisms. In-vivo, Vicia faba plants were exposed to 50 and 100 μM Cr (VI) for 48 h. In-silico, molecular docking and molecular dynamics simulation were used to study the interactions between dichromate and tubulin tyrosine ligase T2R-TTL (PDBID: 5XIW) with reference to Colchicine (microtubule inhibitor). According to our results, Cr (VI) affects growth and cell division and also induces many mitosis aberrations such as chromosome sticking, anaphase/telophase bridges, lagging chromosomes and fragmentation during all phases of mitosis. On the one hand, Cr (VI) reduces mitotic index and promotes micronuclei induction. The in-silico results showed that dichromate establishes very strong bonds at the binding site of the tubulin tyrosine ligase T2R-TTL, with a binding affinity of −5.17 Kcal/Mol and an inhibition constant of 163.59 μM. These interactions are similar to those of colchicine with this protein, so dichromate could be a very potent inhibitor of this protein's activity. TTL plays a fundamental role in the tyrosination/detyrosination of tubulin, which is crucial to the regulation of the microtubule cytoskeleton. Its inhibition leads to the appearance of many morphogenic abnormalities such as mitosis aberrations. In conclusion, our data confirm the highest genotoxicity effects of Cr (VI) on Vicia faba root-cells. •Exposure of Vicia faba L. root cell tips to Cr (VI) resulted in several chromosomal aberrations and abnormalities.•Dichromate may be a very potent inhibitor of tubulin tyrosine ligase's activity.•Dichromate establishes a very strong bond with the binding site of the tubulin tyrosine ligase T2R-TTL.•Cr (VI) can interact with the microtubules of Vicia faba meristematic cell roots and prevents their function.
ISSN:0981-9428
1873-2690
DOI:10.1016/j.plaphy.2024.108361