Selenium mitigates methotrexate‐induced testicular injury: Insights from male NMRI mice model

Background and Aim Chemotherapy, particularly with methotrexate (MTX), often elicits testicular toxicity, leading to impaired spermatogenesis and hormone imbalances. This study aimed to investigate the potential protective effects of selenium (Se) against MTX‐induced testicular injury. Materials and...

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Bibliographic Details
Published in:Birth defects research 2024-02, Vol.116 (2), p.e2315-n/a
Main Authors: Gholami, Mohammadreza, Nemati, Afsaneh, Zarasvand, Azita Alasvand, Zendehdel, Abolfazl, Jalili, Cyrus, Rashidi, Iraj, Mansouri, Kamran, Taheri, Forough, Assadollahi, Vahideh, Gholami, Elham
Format: Article
Language:English
Subjects:
Animals
Apoptosis
BAX protein
Bcl-2 protein
bcl-2-Associated X Protein - metabolism
Bcl-x protein
Biochemical analysis
Biochemical tests
Biopsy
Caspase 3 - metabolism
Caspase-3
Chemotherapy
Damage
Epithelium
Fertility
Follicle Stimulating Hormone
Gene expression
Genes
Injuries
Luteinizing hormone
Luteinizing Hormone - metabolism
Lymphoma
Male
male reproduction
Males
Malondialdehyde
Malondialdehyde - metabolism
Methotrexate
Methotrexate - adverse effects
Mice
Oxidative stress
p53 Protein
Pharmacology
Polymerase chain reaction
Proteins
Selenium
Selenium - pharmacology
Spermatogenesis
Statistical analysis
Structural integrity
Testes
Testosterone
Toxicity
Tumor Suppressor Protein p53
Variance analysis
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Summary:Background and Aim Chemotherapy, particularly with methotrexate (MTX), often elicits testicular toxicity, leading to impaired spermatogenesis and hormone imbalances. This study aimed to investigate the potential protective effects of selenium (Se) against MTX‐induced testicular injury. Materials and Methods Male mice were divided into control, MTX, Se, and MTX + Se groups. Histopathological examination involved the preparation of testicular tissue sections using the Johnsen's tubular biopsy score (JTBS) for spermatogenesis evaluation. Biochemical tests included the assessment of testosterone, malondialdehyde (MDA), luteinizing hormone (LH), and follicle‐stimulating hormone (FSH) levels. Real‐time quantitative polymerase chain reaction (RT‐qPCR) was employed to analyze the expression of caspase 3 (casp3), tumor protein 53 (p53), B‐cell lymphoma 2 (Bcl2), and Bcl2‐associated X protein (Bax) genes. Statistical analysis was performed using ANOVA and Tukey's tests (p 
ISSN:2472-1727
2472-1727
DOI:10.1002/bdr2.2315