High-throughput screening of transient receptor potential (TRP) channels in pterygium

Purpose Pterygium is a hyaline degenerative disease of the conjunctiva characterized by the progression of fibrovascular connective tissue from the bulbar conjunctiva to the cornea. The mechanism of pterygium formation is still not fully understood. Transient receptor potential (TRP) channels are a...

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Bibliographic Details
Published in:International ophthalmology 2024-02, Vol.44 (1), p.63-63, Article 63
Main Authors: Tuylu, Yusuf, Okumus, Seydi, Gul, Rauf, Erbagci, Ibrahim
Format: Article
Language:English
Subjects:
Age composition
Capsaicin receptors
Conjunctiva
Conjunctiva - abnormalities
Conjunctiva - metabolism
Connective tissue
Connective tissue diseases
Connective tissues
Cornea
Epidermal growth factor
Fibroblasts
Gene expression
Genes
High-throughput screening
High-Throughput Screening Assays
Humans
Ion channels
Medicine
Medicine & Public Health
Ophthalmology
Original Paper
Oxidative stress
Pathogenesis
Pterygium - diagnosis
Receptors
Statistical analysis
Therapeutic applications
Transient Receptor Potential Channels - genetics
Transient Receptor Potential Channels - metabolism
Transient receptor potential proteins
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Summary:Purpose Pterygium is a hyaline degenerative disease of the conjunctiva characterized by the progression of fibrovascular connective tissue from the bulbar conjunctiva to the cornea. The mechanism of pterygium formation is still not fully understood. Transient receptor potential (TRP) channels are a group of ion channels with distinct characteristics. Recent indications suggest TRP channels may play a significant regulatory role in pterygium development, but previous studies have mainly focused on in silico analysis. Accordingly, in the present study, we aimed to decipher the expression signatures and role of TRP channels in pterygium development. Methods The study encompassed a cohort of 45 patients matched for age and gender distribution, comprising 30 individuals with primary pterygium (PP) and 15 individuals with recurrent pterygium (RP). The control group consisted of unaffected conjunctival tissue obtained from the same set of patients. High-throughput screening of differentially expressed TRP channels in pterygium tissues was achieved with the help of Fluidigm 96.96 Dynamic Array Expression Chip and reactions were held in BioMark™ HD System Real-Time PCR platform. Results Statistically significant increases were found in the expression of 21 genes, mainly TRPA1 ( p  = 0.021), TRPC2 ( p  = 0.001), and TRPM8 ( p  = 0.003), in patients with PP, and in TRPC5 ( p  = 0.05), TRPM2 ( p  = 0.029), TRPM4 ( p  = 0.03), TRPM6 ( p  = 0.045), TRPM8 ( p  = 0.038), TRPV1 ( p  = 0.01) and TRPV4 ( p  = 0.025) genes in RP tissues. Conclusion Collectively, TRP channel proteins appear to play pivotal roles in both the development and progression of pterygium, making them promising candidates for future therapeutic interventions in patients afflicted by this condition.
ISSN:1573-2630
0165-5701
1573-2630
DOI:10.1007/s10792-024-02938-9