Expression and characterization of α-1,3-glucanase from Paenibacillus alginolyticus NBRC15375, which is classified into subgroup 2 (minor group) of GH family 87

Bacterial α-1,3-glucanase, classified as glycoside hydrolase (GH) family 87, has been divided into 3 subgroups based on differences in gene sequences in the catalytic domain. The enzymatic properties of subgroups 1 and 3 of several bacteria have been previously investigated and reported; however, th...

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Published in:Bioscience, biotechnology, and biochemistry biotechnology, and biochemistry, 2024-04, Vol.88 (5), p.538-545
Main Authors: Konishi, Yasuhito, Sato, Kaito, Nabetani, Kai, Shirasaka, Norifumi, Fukuta, Yasuhisa
Format: Article
Language:English
Subjects:
Amino Acid Sequence
Catalytic Domain
Cloning, Molecular
Escherichia coli - genetics
Gene Expression
Glycoside Hydrolases - chemistry
Glycoside Hydrolases - genetics
Glycoside Hydrolases - metabolism
Hydrogen-Ion Concentration
Oligosaccharides - metabolism
Paenibacillus - enzymology
Paenibacillus - genetics
Recombinant Proteins - chemistry
Recombinant Proteins - genetics
Recombinant Proteins - metabolism
Substrate Specificity
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Summary:Bacterial α-1,3-glucanase, classified as glycoside hydrolase (GH) family 87, has been divided into 3 subgroups based on differences in gene sequences in the catalytic domain. The enzymatic properties of subgroups 1 and 3 of several bacteria have been previously investigated and reported; however, the chemical characterization of subgroup 2 enzymes has not been previously conducted. The α-1,3-glucanase gene from Paenibacillus alginolyticus NBRC15375 (PaAgl) belonging to subgroup 2 of GH family 87 was cloned and expressed in Escherichia coli. PgAgl-N1 (subgroup 3) and PgAgl-N2 (subgroup 1) from P. glycanilyticus NBRC16188 were expressed in E. coli, and their enzymatic characteristics were compared. The amino acid sequence of PaAgl demonstrated that the homology was significantly lower in other subgroups when only the catalytic domain was compared. The oligosaccharide products of the mutan-degrading reaction seemed to have different characteristics among subgroups 1, 2, and 3 in GH family 87.
ISSN:1347-6947
1347-6947
DOI:10.1093/bbb/zbae014