High-throughput mRNA-seq atlas of human placenta shows vast transcriptome remodeling from first to third trimester

The placenta, composed of chorionic villi, changes dramatically across gestation. Understanding differences in ongoing pregnancies are essential to identify the role of chorionic villi at specific times in gestation and develop biomarkers and prognostic indicators of maternal-fetal health. The norma...

Full description

Saved in:
Bibliographic Details
Published in:Biology of reproduction 2024-05, Vol.110 (5), p.936-949
Main Authors: Gonzalez, Tania L, Wertheimer, Sahar, Flowers, Amy E, Wang, Yizhou, Santiskulvong, Chintda, Clark, Ekaterina L, Jefferies, Caroline A, Lawrenson, Kate, Chan, Jessica L, Joshi, Nikhil V, Zhu, Yazhen, Tseng, Hsian-Rong, Karumanchi, S Ananth, Williams Iii, John, Pisarska, Margareta D
Format: Article
Language:English
Subjects:
Adult
Female
High-Throughput Nucleotide Sequencing
Humans
Placenta - metabolism
Pregnancy
Pregnancy Trimester, First - genetics
Pregnancy Trimester, Third - genetics
RNA, Messenger - genetics
RNA, Messenger - metabolism
Transcriptome
Citations: Items that this one cites
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:The placenta, composed of chorionic villi, changes dramatically across gestation. Understanding differences in ongoing pregnancies are essential to identify the role of chorionic villi at specific times in gestation and develop biomarkers and prognostic indicators of maternal-fetal health. The normative mRNA profile is established using next-generation sequencing of 124 first trimester and 43 third trimester human placentas from ongoing healthy pregnancies. Stably expressed genes (SEGs) not different between trimesters and with low variability are identified. Differential expression analysis of first versus third trimester adjusted for fetal sex is performed, followed by a subanalysis with 23 matched pregnancies to control for subject variability using the same genetic and environmental background. Placenta expresses 14,979 polyadenylated genes above sequencing noise (transcripts per million > 0.66), with 10.7% SEGs across gestation. Differentially expressed genes (DEGs) account for 86.7% of genes in the full cohort [false discovery rate (FDR) 
ISSN:0006-3363
1529-7268
DOI:10.1093/biolre/ioae007