Dispersive solid phase extraction of tacrolimus from biological samples using curcumin and iron-based metal organic frameworks nanocomposite followed by LC-MS/MS determination

Tacrolimus is a potent immunosuppressive drug used in the prevention of tissue rejection. It has a narrow therapeutic index. Therefore, the determination of its concentration in biological fluids like plasma and urine is a very crucial issue. In this research, tacrolimus concentrations in plasma and...

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Published in:Journal of chromatography. B, Analytical technologies in the biomedical and life sciences Analytical technologies in the biomedical and life sciences, 2024-02, Vol.1233, p.123977-123977, Article 123977
Main Authors: Siahi-Shadbad, Mohammadreza, Tayebi Khosroshahi, Hamid, Farajzadeh, Mir Ali, Fathi, Ali Akbar, Afshar Mogaddam, Mohammad Reza, Jouyban, Abolghasem
Format: Article
Language:English
Subjects:
Chromatography, High Pressure Liquid - methods
Chromatography, Liquid
Curcumin
Humans
Liquid Chromatography-Mass Spectrometry
Metal-Organic Frameworks - chemistry
Nanocomposites
Solid Phase Extraction - methods
Tacrolimus
Tandem Mass Spectrometry - methods
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Summary:Tacrolimus is a potent immunosuppressive drug used in the prevention of tissue rejection. It has a narrow therapeutic index. Therefore, the determination of its concentration in biological fluids like plasma and urine is a very crucial issue. In this research, tacrolimus concentrations in plasma and urine samples were determined with a dispersive solid phase extraction procedure coupled to high-performance liquid chromatography-tandem mass spectrometry. For this purpose, a curcumin modified metal-organic framework was synthesized and used in extraction procedure. Tacrolimus was adsorbed onto the sorbent surface with aid of vortexing. Then, the adsorbed tacrolimus was eluted by a suitable solvent. Important parameters in extraction procedure were optimized by "one-variable-at-a-time" approach and reported as below: sorbent amount, 10 mg; sample solution pH, 2; agitation mode, vortexing; adsorption and desorption times, 1 min, and eluent (volume), methanol (200 µL). Under the optimized conditions and according to the International Council for Harmonization guidelines, the validation of the method was performed, and the results showed acceptable accuracy and precision (relative standard deviations ≤14 %), good linearity in a wide range (4-200 ng mL ), and low limits of detection (1.2 ng mL in plasma and 0.34 ng mL in urine) and quantification (4.7 ng mL in plasma and 1.12 ng mL in urine). Finally, the validated method was successfully applied for the determination of tacrolimus in the plasma samples of the patients.
ISSN:1570-0232
1873-376X
DOI:10.1016/j.jchromb.2023.123977