Adenoviral Gene Delivery from Multilayered Polyelectrolyte Architectures

The alternate layer‐by‐layer (LBL) deposition of polycations and polyanions for the build up of multilayered polyelectrolyte films is an original approach that allows the preparation of tunable, biologically active surfaces. The resulting supramolecular nanoarchitectures can be functionalized with d...

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Bibliographic Details
Published in:Advanced functional materials 2007-01, Vol.17 (2), p.233-245
Main Authors: Dimitrova, M., Arntz, Y., Lavalle, P., Meyer, F., Wolf, M., Schuster, C., Haïkel, Y., Voegel, J.-C., Ogier, J.
Format: Article
Language:English
Subjects:
Gene delivery
Layer-by-layer assembly
Polyelectrolytes
Thin films
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Summary:The alternate layer‐by‐layer (LBL) deposition of polycations and polyanions for the build up of multilayered polyelectrolyte films is an original approach that allows the preparation of tunable, biologically active surfaces. The resulting supramolecular nanoarchitectures can be functionalized with drugs, peptides, and proteins, or DNA molecules that are able to transfect cells in vitro. We monitor, for the first time, the embedding of a bioactive adenoviral (Ad) vector in multilayered polyelectrolyte films. Ad efficiently adsorbs on poly(L‐lysine)–poly(L‐glutamic acid) (PLL–PGA), PLL–HA (HA: hyaluronan), poly(allylamin hydrochloride)–poly(sodium‐4‐styrenesulfonate) (PAH–PSS), and CHI–HA (CHI: chitosan) films; it preserves its transduction capacity (which can reach 95 %) for a large number of cell types, and also allows vector uptake into receptor‐deficient cells, thus abrogating the restricted tropism of Ad. A bioactive adenoviral vector is embedded into multilayered polyelectrolyte films. Ad efficiently adsorbs on poly(L‐lysine)–poly(L‐glutamic acid), poly(L‐lysine)–hyaluronan, poly(allylamin hydrochloride)–poly(sodium‐4‐styrenesulfonate) (see the AFM image), and chitosan–hyaluronan films, and preserves its transduction capacity for a large number of cell types.
ISSN:1616-301X
1616-3028
DOI:10.1002/adfm.200600155