Sweet diterpenic glycosides balance of a new cultivar of Stevia rebaudiana (Bert.) Bertoni: Isolation and quantitative distribution by chromatographic, spectroscopic, and electrophoretic methods

A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and β-sistosterol were obtained from the former. Stevi...

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Bibliographic Details
Published in:Process biochemistry (1991) 2005-11, Vol.40 (11), p.3587-3594
Main Authors: Dacome, Antonio S., da Silva, Cleuza C., da Costa, Cecília E.M., Fontana, José D., Adelmann, Juliana, da Costa, Silvio Cláudio
Format: Article
Language:English
Subjects:
HPLC
Rebaudioside-A
Stevia rebaudiana
Stevioside
Sweeteners
TLC
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Summary:A dichloromethane fraction and a ultrafiltered water extract of leaves of a new cultivar of Stevia rebaudiana were submitted to separated downstreaming fractionation in silica gel columns. Non-glycosylated and glycosylated pairs for stigmasterol and β-sistosterol were obtained from the former. Stevioside and rebaudioside-A were resolved from the second hydrophilic extract. The structures of these natural compounds were then confirmed through gas chromatography coupled to mass spectrometry and hydrogen/carbon nuclear magnetic resonance. The main sweetener glycoside pair, following scaled-up water extraction, and sugar derivatives downstreaming by membrane filtration and ionic resin exchange, was analyzed by thin layer densitometered chromatography, high-pressure liquid chromatography, and capillary electrophoresis. Thus, an unusual sweetener ratio on the behalf of rebaudioside-A, a glycoside almost devoid of bitter after-taste, was found for a new Stevia cultivar (UEM-320) as compared to other native cultivars, where the bitterer stevioside was the major diterpenic glycoside.
ISSN:1359-5113
1873-3298
DOI:10.1016/j.procbio.2005.03.035