Metabolic Remodulation of Chassis and Corn Stover Bioprocessing to Unlock 3‑Hydroxypropionic Acid Biosynthesis from Agrowaste-Derived Substrates

Embracing the principles of sustainable development, the valorization of agrowastes into value-added chemicals has nowadays received significant attention worldwide. Herein, Escherichia coli was metabolically rewired to convert cellulosic hydrolysate of corn stover into a key platform chemical, name...

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Published in:Journal of agricultural and food chemistry 2024-02, Vol.72 (5), p.2536-2546
Main Authors: Zhou, Lei, Zhang, Yufei, Chen, Tingting, Yun, Junhua, Zhao, Mei, Zabed, Hossain M., Zhang, Cunsheng, Qi, Xianghui
Format: Article
Language:English
Subjects:
Agricultural and Environmental Chemistry
Escherichia coli - genetics
Fermentation
Glycerol - metabolism
Lactic Acid - analogs & derivatives
Metabolic Engineering
Zea mays - metabolism
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Summary:Embracing the principles of sustainable development, the valorization of agrowastes into value-added chemicals has nowadays received significant attention worldwide. Herein, Escherichia coli was metabolically rewired to convert cellulosic hydrolysate of corn stover into a key platform chemical, namely, 3-hydroxypropionic acid (3-HP). First, the heterologous pathways were introduced into E. coli by coexpressing glycerol-3-P dehydrogenase and glycerol-3-P phosphatase in both single and fusion (gpdp12) forms, making the strain capable of synthesizing glycerol from glucose. Subsequently, a glycerol dehydratase (DhaB123-gdrAB) and an aldehyde dehydrogenase (GabD4) were overexpressed to convert glycerol into 3-HP. A fine-tuning between glycerol synthesis and its conversion into 3-HP was successfully established by 5′-untranslated region engineering of gpdp12 and dhaB123-gdrAB. The strain was further metabolically modulated to successfully prevent glycerol flux outside the cell and into the central metabolism. The finally remodulated chassis produced 32.91 g/L 3-HP from the cellulosic hydrolysate of stover during fed-batch fermentation.
ISSN:0021-8561
1520-5118
DOI:10.1021/acs.jafc.3c08419