Dual assistance of surfactants in glycerol organosolv pretreatment and enzymatic hydrolysis of lignocellulosic biomass for bioethanol production

[Display omitted] •Surfactant in glycerol pretreatment facilitated the enzymatic hydrolysis of biomass.•PEG 4000 in pretreatment saved 18.8% energy input with a similar glucose yield.•Coupling surfactants in pretreatment and enzymolysis reduced enzyme usage by 63%•The proposed strategy afforded 1.4 ...

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Bibliographic Details
Published in:Bioresource technology 2024-03, Vol.395, p.130358-130358, Article 130358
Main Authors: Song, Guojie, Sun, Chihe, Madadi, Meysam, Dou, Shaohua, Yan, Junshu, Huan, Hailin, Aghbashlo, Mortaza, Tabatabaei, Meisam, Sun, Fubao, Ashori, Alireza
Format: Article
Language:English
Subjects:
Biomass
Cellulose - metabolism
Cellulosic ethanol
Economic analysis
Fermentation
Glucose
Glycerol
High-solid enzymatic hydrolysis
Hydrolysis
Sugarcane bagasse
Surface-Active Agents
Surfactant-assisted pretreatment
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Summary:[Display omitted] •Surfactant in glycerol pretreatment facilitated the enzymatic hydrolysis of biomass.•PEG 4000 in pretreatment saved 18.8% energy input with a similar glucose yield.•Coupling surfactants in pretreatment and enzymolysis reduced enzyme usage by 63%•The proposed strategy afforded 1.4 MJ/kg of net energy gain for ethanol production. This study investigated an innovative strategy of incorporating surfactants into alkaline-catalyzed glycerol pretreatment and enzymatic hydrolysis to improve lignocellulosic biomass (LCB) conversion efficiency. Results revealed that adding 40 mg/g PEG 4000 to the pretreatment at 195 °C obtained the highest glucose yield (84.6%). This yield was comparable to that achieved without surfactants at a higher temperature (240 °C), indicating a reduction of 18.8% in the required heat input. Subsequently, Triton X-100 addition during enzymatic hydrolysis of PEG 4000-assisted pretreated substrate increased glucose yields to 92.1% at 6 FPU/g enzyme loading. High-solid fed-batch semi-simultaneous saccharification and co-fermentation using this dual surfactant strategy gave 56.4 g/L ethanol and a positive net energy gain of 1.4 MJ/kg. Significantly, dual assistance with surfactants rendered 56.3% enzyme cost savings compared to controls without surfactants. Therefore, the proposed surfactant dual-assisted promising approach opens the gateway to economically viable enzyme-mediated LCB biorefinery.
ISSN:0960-8524
1873-2976
DOI:10.1016/j.biortech.2024.130358