Single-atom iron boosts electrochemiluminescence for ultrasensitive carcinoembryonic antigen detection

A simple and ultrasensitive sandwich-type electrochemiluminescence (ECL) immunosensor has been developed using porous three-dimensional gold nanoparticles (Au NPs) iron(Fe)–zinc(Zn) metal–organic frameworks (Au NPs–FeZn–MOFs@luminol) as high-efficiency ECL signal probes with Fe single-atom catalysts...

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Bibliographic Details
Published in:Mikrochimica acta (1966) 2024-02, Vol.191 (2), p.111-111, Article 111
Main Authors: Huang, Xiaomei, Deng, Haoxuan, Deng, Xiang, Li, Longxiang, Wu, Mao, Huang, Chaoqin, Zhang, Yuxing, Zhao, Huali
Format: Article
Language:English
Subjects:
Analytical Chemistry
Anions
Antigens
Biosensing Techniques
Carcinoembryonic Antigen
Characterization and Evaluation of Materials
Chemical reduction
Chemistry
Chemistry and Materials Science
Dissolved oxygen
Electrochemical oxidation
Electrochemiluminescence
Electronic structure
Electrons
Gold
Hydrogen peroxide
Immunoassay
Immunosensors
Iron
Luminol
Metal Nanoparticles
Metal-organic frameworks
Microengineering
Nanochemistry
Nanoparticles
Nanotechnology
Original Paper
Oxygen reduction reactions
Radicals
Reactive Oxygen Species
Single atom catalysts
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Summary:A simple and ultrasensitive sandwich-type electrochemiluminescence (ECL) immunosensor has been developed using porous three-dimensional gold nanoparticles (Au NPs) iron(Fe)–zinc(Zn) metal–organic frameworks (Au NPs–FeZn–MOFs@luminol) as high-efficiency ECL signal probes with Fe single-atom catalysts (SACs) (Fe–N–C SACs) as potentially advanced coreaction accelerators and dissolved oxygen as a coreaction agent to realize an H 2 O 2 -free amplification method for detecting carcinoembryonic antigen (CEA). The cathodic ECL of luminol, which was usually negligible, increased first. Because the Fe–N–C SACs exhibited an outstanding catalytic performance and a unique electronic structure, different reactive oxygen species (ROS) were generated via the oxygen reduction reaction. ROS oxidized the luminol anions to luminol anion radicals, preventing the time-consuming luminol electrochemical oxidation. Furthermore, the luminol anion radicals generated in situ reacted with ROS to produce potent cathodic ECL emissions. The immunosensor exhibited favorable analytical accuracy (detection range: 0.1 pg mL −1  – 80 ng mL −1 ), and its detection limit for serum samples was 0.031 pg mL −1 (S/N = 3). Consequently, the proposed strategy offers a new approach for early screening of CEA. Graphical abstract
ISSN:0026-3672
1436-5073
DOI:10.1007/s00604-024-06188-5