Intramuscular therapeutic doses of enrofloxacin affect microbial community structure but not the relative abundance of fluoroquinolones resistance genes in swine manure

Livestock manure is a major source of veterinary antibiotics and antibiotic resistance genes (ARGs). Elucidation of the residual characteristics of ARGs in livestock manure following the administration of veterinary antibiotics is critical to assess their ecotoxicological effects and environmental c...

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Published in:The Science of the total environment 2024-02, Vol.913, p.169794-169794, Article 169794
Main Authors: Li, Xianghui, Tang, Xiaoyue, Chen, Majan, Wang, Shaoyu, Tong, Chang, Xu, Jiaojiao, Xie, Gaomiao, Ma, Baohua, Zou, Yongde, Wang, Yan, Wen, Xin, Wu, Yinbao
Format: Article
Language:English
Subjects:
Animals
Anti-Bacterial Agents - pharmacology
Composting
Enrofloxacin
Fluoroquinolones
Fluoroquinolones resistance genes
Genes, Bacterial
Livestock
Manure - microbiology
Metagenomics
Microbial community structure
Microbiota
Swine
Swine manure
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Summary:Livestock manure is a major source of veterinary antibiotics and antibiotic resistance genes (ARGs). Elucidation of the residual characteristics of ARGs in livestock manure following the administration of veterinary antibiotics is critical to assess their ecotoxicological effects and environmental contamination risks. Here, we investigated the effects of enrofloxacin (ENR), a fluoroquinolone antibiotic commonly used as a therapeutic drug in animal husbandry, on the characteristics of ARGs, mobile genetic elements, and microbial community structure in swine manure following its intramuscular administration for 3 days and a withdrawal period of 10 days. The results revealed the highest concentrations of ENR and ciprofloxacin (CIP) in swine manure at the end of the administration period, ENR concentrations in swine manure in groups L and H were 88.67 ± 45.46 and 219.75 ± 88.05 mg/kg DM, respectively. Approximately 15 fluoroquinolone resistance genes (FRGs) and 48 fluoroquinolone-related multidrug resistance genes (F-MRGs) were detected in swine manure; the relative abundance of the F-MRGs was considerably higher than that of the FRGs. On day 3, the relative abundance of qacA was significantly higher in group H than in group CK, and no significant differences in the relative abundance of other FRGs, F-MRGs, or MGEs were observed between the three groups on day 3 and day 13. The microbial community structure in swine manure was significantly altered on day 3, and the altered community structure was restored on day 13. The FRGs and F-MRGs with the highest relative abundance were qacA and adeF, respectively, and Clostridium and Lactobacillus were the dominant bacterial genera carrying these genes in swine manure. In summary, a single treatment of intramuscular ENR transiently increased antibiotic concentrations and altered the microbial community structure in swine manure; however, this treatment did not significantly affect the abundance of FRGs and F-MRGs. [Display omitted] •F-MRGs had a higher number and relative abundance than FRGs in swine manure.•Intramuscular ENR transiently altered the microbial community structure.•Intramuscular ENR unaffected relative abundance of F-MRGs and MGEs.•Intramuscular high doses of ENR alter the relative abundance of qacA on day3.•Host bacteria of FRGs and F-MRGs were the dominant genera in swine manure.
ISSN:0048-9697
1879-1026
DOI:10.1016/j.scitotenv.2023.169794