The presence of free palmitic acid modulates the effects of lutein on structural and dynamic properties of lipid membranes

Free fatty acids, like palmitic acid (PA), and xanthophyll pigments, like lutein (LUT) are the natural membrane compounds in plants. To study the effect of PA on LUT and their organization, a model membrane of 1,2-dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) enriched with 2 mol% PA and 1 mol% LUT...

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Published in:Archives of biochemistry and biophysics 2024-02, Vol.752, p.109883-109883, Article 109883
Main Authors: Reszczyńska, Emilia, Wiśniewska-Becker, Anna, Duda, Mariusz, Sęk, Alicja, Gruszecki, Wiesław I, Hanaka, Agnieszka
Format: Article
Language:English
Subjects:
Dimyristoylphosphatidylcholine - chemistry
Electron Spin Resonance Spectroscopy
Humans
Lipid Bilayers - chemistry
Lipids
Lutein - chemistry
Lutein - pharmacology
Palmitic Acids
Xanthophylls
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Summary:Free fatty acids, like palmitic acid (PA), and xanthophyll pigments, like lutein (LUT) are the natural membrane compounds in plants. To study the effect of PA on LUT and their organization, a model membrane of 1,2-dimyristoyl-sn-glycerol-3-phosphocholine (DMPC) enriched with 2 mol% PA and 1 mol% LUT was formed. Molecular mechanisms underlying the interaction between these two compounds were examined with application of molecular spectroscopy techniques, e.g., visible spectroscopy, electron paramagnetic resonance and Fourier transform infrared. We determined the monomeric/dimeric organization of LUT in the membrane. We proved that the presence of PA in the lipid phase facilitated and stabilized the formation of LUT structures in the membrane. Lutein with PA did not form strong molecular aggregates like H- and J-structures. We presented the simplified model membrane that could be a suitable representation of the physiological process of de-esterification of PA from LUT appearing in natural biomembranes in humans.
ISSN:0003-9861
1096-0384
DOI:10.1016/j.abb.2024.109883