Glycolate as alternative carbon source for Escherichia coli

The physiology of different Escherichia coli stains was analyzed for growth with glycolate as a potentially promising sustainable sole source of carbon and energy. Different E. coli strains showed large differences regarding lag phases after provision of glycolate. Whereas E. coli W showed fast adap...

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Bibliographic Details
Published in:Journal of biotechnology 2024-02, Vol.381, p.76-85
Main Authors: Höhmann, Sonja, Briol, Tim Arik, Ihle, Nadine, Frick, Oliver, Schmid, Andreas, Bühler, Bruno
Format: Article
Language:English
Subjects:
Alternative carbon source
Carbon - metabolism
Escherichia coli - genetics
Escherichia coli - metabolism
Glycolate utilization
Glycolates - metabolism
Glycolic acid
Laboratory adaption
Physiology
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Summary:The physiology of different Escherichia coli stains was analyzed for growth with glycolate as a potentially promising sustainable sole source of carbon and energy. Different E. coli strains showed large differences regarding lag phases after provision of glycolate. Whereas E. coli W showed fast adaptation, E. coli BW25113, JM101, and BL21 (DE3) needed extensive time for adaption (up to 30 generations) until the attainable µmax was reached, which, at 30 °C, amounted to 0.20–0.25 h−1 for all strains. The overexpression of genes encoding glycolate degradation did neither overcome the need for adaptation of E. coli BL21 (DE3) nor improve growth of E. coli W. Rather, high level expression of proteins involved in uptake and initial degradation steps had an adverse effect on growth. Overall, the results show a promising capacity of E. coli strains for growth on glycolate. [Display omitted] •E. coli strains differ regarding adaptation duration for growth on glycolate•Adapted E. coli strains showed efficient growth on glycolate•Overexpression of early degradation genes impairs growth on glycolate.
ISSN:0168-1656
1873-4863
DOI:10.1016/j.jbiotec.2024.01.001