Necrosis, netosis, and apoptosis in pulmonary tuberculosis and type-2 diabetes mellitus. Clues from the patient's serum

Pulmonary tuberculosis (PTB) and type 2 diabetes mellitus (T2DM) are two inflammatory diseases whose pathology involves neutrophils (NEU) as key participants. Countless inflammatory elements produced at the lesion sites leak into the blood and are distributed systemically. The study aimed to investi...

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Published in:Tuberculosis (Edinburgh, Scotland) Scotland), 2023-12, Vol.143, p.102426-102426, Article 102426
Main Authors: Rojas-Espinosa, Oscar, Arce-Mendoza, Alma Yolanda, Islas-Trujillo, Sergio, Muñiz-Buenrostro, Antonio, Arce-Paredes, Patricia, Popoca-Galván, Omar, Moreno-Altamirano, Bertha, Rivero Silva, Miguel
Format: Article
Language:English
Subjects:
Apoptosis
Coloring Agents
Diabetes
Diabetes Mellitus, Type 2 - diagnosis
Humans
Mycobacterium tuberculosis
Necrosis
Netosis
Tuberculosis
Tuberculosis, Pulmonary
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Summary:Pulmonary tuberculosis (PTB) and type 2 diabetes mellitus (T2DM) are two inflammatory diseases whose pathology involves neutrophils (NEU) as key participants. Countless inflammatory elements produced at the lesion sites leak into the blood and are distributed systemically. The study aimed to investigate the effect of the serum of patients with PTB, T2DM, and PTB + T2DM on the cellular and nuclear morphology of healthy NEU. Monolayers of NEU were prepared and incubated with sera from PTB (n꓿ 10), T2DM (n꓿10), PTB + T2DM (n꓿ 10) patients, or sera from healthy people (n = 10). Monolayers were stained for histones, elastase, and myeloperoxidase for NETosis, annexin V for apoptosis, and Iris fuchsia for necrosis. Hoechst stain (DNA) was used to identify the nuclear alterations. Necrosis was the predominant alteration. Sera from PTB + T2DM were the most potent change inducers. Normal sera did not induce cell alterations. The blood of TBP and T2DM patients carries a myriad of abnormal elements that induce necrosis of NEU in normal people, thus reflecting what might occur in the neutrophils of the patients themselves. These findings reinforce the participation of NEU in the pathology of these diseases. Necrosis is expected to be the most frequent neutrophil-induced alteration in tuberculosis and diabetes mellitus. •Pulmonary tuberculosis and type 2 diabetes mellitus are chronic diseases where inflammation is a prime component.•Infectious and non-infectious inflammation have neutrophils as key participants.•Early inflammation in tuberculosis and diabetes plays a beneficial role but an adverse role if inflammation is sustained.•Blood in patients with chronic tuberculous or diabetes carries a myriad of dangerous elements that affect the physiology of neutrophils, compelling them to behave as pathogenic elements.•Neutrophilia in most inflammatory diseases correlates with poor prognosis because of the high toxicity of neutrophils.
ISSN:1472-9792
1873-281X
DOI:10.1016/j.tube.2023.102426