Structural characterization of an inulin neoseries-type fructan from Ophiopogonis Radix and the therapeutic effect on liver fibrosis in vivo

Ophiopogonis Radix is a well-known Traditional Chinese Medicine and functional food that is rich in polysaccharides and has fructan as a characteristic component. In this study, an inulin neoseries-type fructan designated as OJP-W2 was obtained and characterized from Ophiopogonis Radix, and its pote...

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Published in:Carbohydrate polymers 2024-03, Vol.327, p.121659-121659, Article 121659
Main Authors: Liu, Wei, Zhang, Linzhang, Wei, Xia, Xu, Yongbin, Fang, Qinqin, Qi, Shenglan, Chen, Jiamei, Wang, Changhong, Wang, Shunchun, Qin, Luping, Liu, Ping, Wu, Jianjun
Format: Article
Language:English
Subjects:
Fructan
Fructans - chemistry
Fructans - pharmacology
Fructans - therapeutic use
Glucose
Humans
Inulin - pharmacology
Inulin - therapeutic use
Liver Cirrhosis - drug therapy
Liver fibrosis
Ophiopogonis radix
Polysaccharides
Structural characterization
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Summary:Ophiopogonis Radix is a well-known Traditional Chinese Medicine and functional food that is rich in polysaccharides and has fructan as a characteristic component. In this study, an inulin neoseries-type fructan designated as OJP-W2 was obtained and characterized from Ophiopogonis Radix, and its potential therapeutic effect on liver fibrosis in vivo were investigated. Structural studies revealed that OJP-W2 had a molecular weight of 5.76 kDa and was composed of glucose and fructose with a molar ratio of 1.00:30.87. Further analysis revealed OJP-W2 has a predominantly lineal (1–2)-linked β-D-fructosyl units linked to the glucose moiety of the sucrose molecule with (2–6)-linked β-D-fructosyl side chains. Pharmacological studies revealed that OJP-W2 exerted a marked hepatoprotective effect against liver fibrosis, the mechanism of action was involved in regulating collagen deposition (α-SMA, COL1A1 and liver Hyp contents) and TGF-β/Smads signaling pathway, alleviating liver inflammation (IL-1β, IL-6, CCL5 and F4/80) and MAPK signaling pathway, and inhibiting hepatic apoptosis (Bax, Bcl-2, ATF4 and Caspase 3). These data provide evidence for expanding Ophiopogonis Radix-acquired fructan types and advancing our understanding of the specific role of inulin neoseries-type fructan in liver fibrosis therapy. [Display omitted]
ISSN:0144-8617
1879-1344
DOI:10.1016/j.carbpol.2023.121659