Preparation of a novel MOF–POPM and its application in online purification and enrichment of oleanolic acid in medicinal plants

In present work, a method for enrichment, purification, and content determination of oleanolic acid (OA) in medicinal plants was established based on on-line solid phase extraction (SPE). A metal organic frameworks–porous organic polymer monolith (MOF–POPM) was prepared with functionalized UiO-66-(O...

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Bibliographic Details
Published in:Analytical sciences 2024-02, Vol.40 (2), p.319-333
Main Authors: Zhang, Miaomiao, Wei, Xuanwen, Bai, Ligai, Liu, Haiyan
Format: Article
Language:English
Subjects:
Analytical Chemistry
Chemistry
Chemistry and Materials Science
Chromatography, High Pressure Liquid - methods
Oleanolic Acid
Original Paper
Plants, Medicinal
Polymers - chemistry
Solid Phase Extraction - methods
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Summary:In present work, a method for enrichment, purification, and content determination of oleanolic acid (OA) in medicinal plants was established based on on-line solid phase extraction (SPE). A metal organic frameworks–porous organic polymer monolith (MOF–POPM) was prepared with functionalized UiO-66-(OH) 2 as monomer and was used as SPE column for online enrichment and purification of OA. The ratio of adsorbent, enriching and eluting solvent, mobile phase pH, and flow rate had been systematically investigated. Under the optimum conditions, the linear range of OA was 0.59–2500 μg/mL with r  = 0.9996. The limit of detection (LOD) was 0.18 μg/mL and the limit of quantification (LOQ) was 0.59 μg/mL. The intra-day relative standard deviations (RSDs) and inter-day RSDs of retention time and peak area were less than 0.3% and 1.3%, respectively. The average recoveries of OA in medicinal plants samples ranged from 87.7 to 104.6%. The results demonstrated that the online system was reliable and accurate for enrichment, purification, and content determination of OA in medicinal plants. Graphical abstract
ISSN:0910-6340
1348-2246
DOI:10.1007/s44211-023-00465-8