Changes in neuroinflammatory markers and microglial density in the hippocampus and prefrontal cortex of the C58/J mouse model of autism

Autism spectrum disorder (ASD) is a diverse group of neurodevelopmental conditions with complex origins. Individuals with ASD present various neurobiological abnormalities, including an altered immune response in the central nervous system and other tissues. Animal models like the C58/J inbred mouse...

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Bibliographic Details
Published in:The European journal of neuroscience 2024-01, Vol.59 (1), p.154-173
Main Authors: Duarte‐Campos, Juan F., Vázquez‐Moreno, C. Noé, Martínez‐Marcial, Mónica, Chavarría, Anahí, Ramírez‐Carreto, Ricardo Jair, Velasco Velázquez, Marco A., De La Fuente‐Granada, Marisol, González‐Arenas, Aliesha
Format: Article
Language:English
Subjects:
Animal models
Arginase
Autism
autism spectrum disorder
Central nervous system
CX3CR1 protein
Dendritic spines
Fractalkine
Hippocampus
Immune response
Inbreeding
Inflammation
Interferon
microglia
Monocyte chemoattractant protein
Monocyte chemoattractant protein 1
Monocytes
neuroinflammation
Prefrontal cortex
proinflammatory cytokines
Social behavior
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Summary:Autism spectrum disorder (ASD) is a diverse group of neurodevelopmental conditions with complex origins. Individuals with ASD present various neurobiological abnormalities, including an altered immune response in the central nervous system and other tissues. Animal models like the C58/J inbred mouse strain are used to study biological characteristics of ASD. This strain is considered an idiopathic autism model because of its demonstrated reduced social preference and repetitive behaviours. Notably, C58/J mice exhibit alterations in dendritic arbour complexity, density and dendritic spines maturation in the hippocampus and prefrontal cortex (PFC), but inflammatory‐related changes have not been explored in these mice. In this study, we investigated proinflammatory markers in the hippocampus and PFC of adult male C58/J mice. We discovered elevated levels of interferon gamma (IFN‐γ) and monocyte chemoattractant protein 1 (MCP‐1) in the hippocampus, suggesting increased inflammation, alongside a reduction in the anti‐inflammatory enzyme arginase 1 (ARG1). Conversely, the PFC displayed reduced levels of TNF‐α and MCP‐1. Microglial analysis revealed higher levels of transmembrane protein 119 (TMEM119) and increased microglial density in a region‐specific manner of the autistic‐like mice, particularly in the PFC and hippocampus. Additionally, an augmented expression of the fractalkine receptor CX3CR1 was observed in the hippocampus and PFC of C58/J mice. Microglial morphological analysis shows no evident changes in the hippocampus of mice with autistic‐like behaviours versus wild‐type strain. These region‐specific changes can contribute to modulate processes like inflammation or synaptic pruning in the C58/J mouse model of idiopathic autism. Our study highlights region‐specific neuroinflammatory differences in C58/J mice, an idiopathic model of autism. The hippocampus shows increased microglial density and inflammatory mediators, whereas the prefrontal cortex exhibits a trend toward increased microglial density and decreased inflammatory mediators. These findings imply distinct inflammatory and microglial activity modulation in C58/J mice with autistic‐like traits.
ISSN:0953-816X
1460-9568
DOI:10.1111/ejn.16204