Key Challenges for In Vitro Testing of Tobacco Products for Regulatory Applications: Recommendations for the In Vitro Mouse Lymphoma Assay

The Institute for In Vitro Sciences (IIVS) is sponsoring a series of workshops to develop recommendations for optimal scientific and technical approaches for conducting in vitro assays to assess potential toxicity within and across traditional tobacco and various tobacco and nicotine next-generation...

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Bibliographic Details
Published in:Alternatives to laboratory animals 2024-01, Vol.52 (1), p.42-59
Main Authors: Crooks, Ian, Clements, Julie, Curren, Rodger, Guo, Xiaoqing, Hollings, Michael, Lloyd, Mel, Smart, Daniel, Thorne, David, Weber, Elisabeth, Moore, Martha
Format: Article
Language:English
Subjects:
Animals
Electronic Nicotine Delivery Systems
In Vitro Techniques
Mice
Nicotine
Research Design
Tobacco Products - toxicity
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Summary:The Institute for In Vitro Sciences (IIVS) is sponsoring a series of workshops to develop recommendations for optimal scientific and technical approaches for conducting in vitro assays to assess potential toxicity within and across traditional tobacco and various tobacco and nicotine next-generation products (NGPs), including Heated Tobacco Products (HTPs) and Electronic Nicotine Delivery Systems (ENDS). This report was developed by a working group composed of attendees of the seventh IIVS workshop, ‘Approaches and recommendations for conducting the mouse lymphoma gene mutation assay (MLA) and introduction to in vitro disease models’, which was held virtually on 21–23 June 2022. This publication provides a background overview of the MLA, and includes the description of assay conduct and data interpretation, key challenges and recommended best practices for evaluating tobacco and nicotine products, with a focus on the evaluation of NGPs, and a summary of how the assay has been used to evaluate and compare tobacco and nicotine products.
ISSN:0261-1929
2632-3559
DOI:10.1177/02611929231219153