Serological identification of MERS-CoV in camels of Wasit province, Iraq

BackgroundSince the first human case of Middle East Respiratory Syndrome (MERS) caused by Coronavirus (MERS-CoV) in 2012, several evidence bases have shown one-humped camels as the main reservoir host, from which infection is transmitted to humans.AimSerological investigation of MERS in dromedary ca...

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Bibliographic Details
Published in:Open Veterinary Journal 2023-01, Vol.13 (10), p.1283-1289
Main Authors: Al-Taee, Hala S R, Sekhi, Azhar Ali, Gharban, Hasanain A J, Biati, Hussien M A
Format: Article
Language:English
Subjects:
camelus dromedarius
coronavirus
elisa
mers-cov
serological test
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Summary:BackgroundSince the first human case of Middle East Respiratory Syndrome (MERS) caused by Coronavirus (MERS-CoV) in 2012, several evidence bases have shown one-humped camels as the main reservoir host, from which infection is transmitted to humans.AimSerological investigation of MERS in dromedary camels in Wasit province (Iraq), detection severity of infection, and association to some risk factors.MethodsA total of 455 dromedary camels were selected randomly from two main districts in Wasit province, Iraq, during January and April (2023). Sera of all study camels were examined by enzyme-linked immunosorbent assay (ELISA), and titers of positive study animals were categorized according to their severity.ResultsSerological testing yielded 37.58% positive animals for MERS infection. According to the severity of positive ODs (titer), a total of 53.22%, 30.99%, 12.28%, and 3.51% showed mild, moderate, strong, and very strong infections, respectively. Regarding risk factors, significant elevation in seropositivity was seen in camels of >3-6 and >6 years old and reduced in camels of £3 years old with an elevated risk of MERS with increased age. Regionally, seropositivity and relative risk were increased in the camels of Shaykh Sa'd when compared with Al-Numaniyah. Regarding sex, no significant variation was detected between seropositive females and males; however, male camels appeared at higher risk than females. Association between the severity of MERS infection and risk factors revealed that there was a significant increase in mild and moderate infections in female camels of >6 years old; whereas strong and very strong infections were seen in male camels of 33-6 years old. Mild and very strong infections were recorded in Shaykh Sa'd; while moderate and strong infections in Al-Numaniyah.ConclusionThe study indicated a longstanding existence of MERS-CoV in camels of Wasit province; therefore, recent infections or active viral excretion are required for confirmation by molecular approaches.
ISSN:2226-4485
2218-6050
DOI:10.5455/OVJ.2023.v13.i10.7