Long-Term Imaging of Cys in Cells and Tumor Mice by a Solid-State Fluorescence Probe

Cysteine is an important biological thiol and is closely related to cancer. It remains a challenge to develop a probe that can provide long-term fluorescence detection and imaging of Cys in cells as well as in living organisms. Here, a solid-state fluorophore is combined with an acrylate group to co...

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Bibliographic Details
Published in:Analytical chemistry (Washington) 2023-12, Vol.95 (48), p.17559-17567
Main Authors: Fu, Gui-Qin, Song, Qian, Wang, Zhi-Qing, Chao, Jing-Jing, Zhang, Hui, Mao, Guo-Jiang, Chen, Dong-Hua, Li, Chun-Yan
Format: Article
Language:English
Subjects:
Animals
Biocompatibility
Cysteine
Cytotoxicity
Electron transfer
Fluorescence
Fluorescent Dyes - toxicity
Fluorescent indicators
HeLa Cells
Humans
Imaging
Mice
Protons
Solid state
Sulfhydryl Compounds
Tumors
Wavelengths
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Summary:Cysteine is an important biological thiol and is closely related to cancer. It remains a challenge to develop a probe that can provide long-term fluorescence detection and imaging of Cys in cells as well as in living organisms. Here, a solid-state fluorophore is combined with an acrylate group to construct a solid-state fluorescent probe for Cys recognition. The fluorescence of the probe is quenched when the photoinduced electron transfer (PET) process is turned on and the excited-state intramolecular proton transfer (ESIPT) process is turned off. In the presence of Cys, an obvious solid-state fluorescence signal can be observed. The double quenching mechanism makes the probe have the advantages of high sensitivity, good selectivity, and high contrast of biological imaging. Due to low cytotoxicity, the probe can be used to detect exogenous and endogenous Cys in living cells and is capable of imaging over long periods of time. By making full use of long wavelengths, the probe can be applied for the detection of Cys levels in tumor mice and equipped with the ability to conduct long-term imaging in vivo.
ISSN:0003-2700
1520-6882
DOI:10.1021/acs.analchem.3c02872