Exchangeable Self‐Assembled Lanthanide Antennas for PLIM Microscopy

Lanthanides have unique photoluminescence (PL) emission properties, including very long PL lifetimes. This makes them ideal for biological imaging applications, especially using PL lifetime imaging microscopy (PLIM). PLIM is an inherently multidimensional technique with exceptional advantages for qu...

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Bibliographic Details
Published in:Angewandte Chemie International Edition 2024-02, Vol.63 (6), p.e202314595-n/a
Main Authors: Ruiz‐Arias, Alvaro, Fueyo‐González, Francisco, Izquierdo‐García, Carolina, Navarro, Amparo, Gutiérrez‐Rodríguez, Marta, Herranz, Rosario, Burgio, Chiara, Reinoso, Antonio, Cuerva, Juan M., Orte, Angel, González‐Vera, Juan A.
Format: Article
Language:English
Subjects:
Antennas
Bioimaging
Emission
Emissions
Image acquisition
Lanthanide Antenna
Lanthanides
Lanthanoid Series Elements
Luminescence
Medical imaging
Microscopy
Phosphonic acids
Photobleaching
Photochemical reactions
Photoluminescence
Photons
Photostability
PLIM
Quinoline
Self-assembly
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Summary:Lanthanides have unique photoluminescence (PL) emission properties, including very long PL lifetimes. This makes them ideal for biological imaging applications, especially using PL lifetime imaging microscopy (PLIM). PLIM is an inherently multidimensional technique with exceptional advantages for quantitative biological imaging. Unfortunately, due to the required prolonged acquisitions times, photobleaching of lanthanide PL emission currently constitutes one of the main drawbacks of PLIM. In this study, we report a small aqueous‐soluble, lanthanide antenna, 8‐methoxy‐2‐oxo‐1,2,4,5‐tetrahydrocyclopenta[de]quinoline‐3‐phosphonic acid, PAnt, specifically designed to dynamically interact with lanthanide ions, serving as exchangeable dye aimed at mitigating photobleaching in PLIM microscopy in cellulo. Thus, self‐assembled lanthanide complexes that may be photobleached during image acquisition are continuously replenished by intact lanthanide antennas from a large reservoir. Remarkably, our self‐assembled lanthanide complex clearly demonstrated a significant reduction of PL photobleaching when compared to well‐established lanthanide cryptates, used for bioimaging. This concept of exchangeable lanthanide antennas opens new possibilities for quantitative PLIM bioimaging. We report a small aqueous‐soluble lanthanide antenna (PAnt) specifically designed to dynamically interact with lanthanide ions and act as an exchangeable dye, aiming at mitigating photobleaching in PLIM microscopy in cellulo. Our self‐assembled lanthanide complex exhibited an exceptional photostability compared to traditional lanthanide cryptates, marking a significant advance in quantitative PLIM bioimaging.
ISSN:1433-7851
1521-3773
1521-3773
DOI:10.1002/anie.202314595