Tyrosinase enzyme purification and immobilization from Pseudomonas sp. EG22 using cellulose coated magnetic nanoparticles: characterization and application in melanin production

Melanin is a brown-black pigment with significant roles in various biological processes. The tyrosinase enzyme catalyzes the conversion of tyrosine to melanin and has promising uses in the pharmaceutical and biotechnology sectors. This research aims to purify and immobilize the tyrosinase enzyme fro...

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Bibliographic Details
Published in:World journal of microbiology & biotechnology 2024-01, Vol.40 (1), p.10-10, Article 10
Main Authors: El-Aziz, Salim Mohamed Abd, Faraag, Ahmed Hassan Ibrahim, Ibrahim, Ayman Meselhi, Albrakati, Ashraf, Bakkar, Marwa Reda
Format: Article
Language:English
Subjects:
Anti-Bacterial Agents - pharmacology
Antibacterial activity
Apoptosis
Applied Microbiology
Biochemistry
Biological activity
Biomedical and Life Sciences
Biotechnology
Cell survival
Cellulose
Drug resistance
E coli
Environmental Engineering/Biotechnology
Enzymes
Enzymes, Immobilized - chemistry
Escherichia coli
Immobilization
Life Sciences
Magnetite Nanoparticles - chemistry
Melanin
Melanins
Microbiology
Monophenol Monooxygenase
Multidrug resistance
Nanoparticles
Polydispersity
Pseudomonas
Staphylococcus aureus
Tyrosinase
Tyrosine
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Summary:Melanin is a brown-black pigment with significant roles in various biological processes. The tyrosinase enzyme catalyzes the conversion of tyrosine to melanin and has promising uses in the pharmaceutical and biotechnology sectors. This research aims to purify and immobilize the tyrosinase enzyme from Pseudomonas sp . EG22 using cellulose-coated magnetic nanoparticles. Various techniques were utilized to examine the synthesized nanoparticles, which exhibited a spherical shape with an average diameter of 12 nm and a negative surface potential of − 55.7 mV with a polydispersity index (PDI) of 0.260. Comparing the immobilized magnetic tyrosinase enzyme with the free enzyme, the study’s findings showed that the immobilized tyrosinase enzyme had optimal activity at a pH of 6 and a temperature of 35 °C, and its activity increased as the concentration of tyrosine increased. The study investigated the antibacterial and anticancer bioactivity of the enzyme’s melanin product and found that it exhibited potential antibacterial activity against a multi-drug resistant strain including S. aureus and E. coli . The produced melanin also demonstrated the potential to decrease cell survival and induce apoptosis in initiation cells.
ISSN:0959-3993
1573-0972
DOI:10.1007/s11274-023-03796-w