Assessment of Biological Activities, Acute and Sub-chronic Toxicity of Liang (Gnetum gnemon var. tenerum) Leaves Powder, a Natural Product

Assessment of Biological Activities, Acute and Sub-chronic Toxicity of Liang (Gnetum gnemon var. tenerum) Leaves Powder, a Natural Product

Gnetum gnemon var. tenerum (Gnetaceae) is a shrub plant native to South-East Asia. In Thailand, Liang leaves are commonly consumed in South of Thailand as vegetable. According to literature, they have an antihyperglycemic capacity because of their rich chlorophyll, fiber, and protein. However, there...

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Bibliographic Details
Published in:Biological & pharmaceutical bulletin 2023/12/01, Vol.46(12), pp.1666-1675
Main Authors: Suksanga, Anunya, Siripongvutikorn, Sunisa, Leelawattana, Rattana, Yupanqui, Chutha Takahashi, Idowu, Ayodeji Oluwafemi
Format: Article
Language:eng
Subjects:
Acute toxicity
Body weight
Chlorophyll
Chronic toxicity
Creatinine
Erythrocytes
Gnetum gnemon
Hematocrit
Hemoglobin
Inflammation
Kidneys
Leaves
Lethal dose
Liang leaf
Natural products
OECD Guideline 408
Organisation for Economic Co-operation and Development (OECD) Guideline 425
sub-chronic toxicity
Toxicity
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Summary:Gnetum gnemon var. tenerum (Gnetaceae) is a shrub plant native to South-East Asia. In Thailand, Liang leaves are commonly consumed in South of Thailand as vegetable. According to literature, they have an antihyperglycemic capacity because of their rich chlorophyll, fiber, and protein. However, there is need to assess the safety since natural food products are not completely devoid of toxicity. This study aimed to assess the biological activities as well as the acute and sub-chronic oral toxicity of Liang leaves powder (LLP). The evaluation of LLP for acute oral toxicity was performed at dose level 2000 mg/kg body weight in Wistar rats while the sub-chronic oral toxicity of LLP was performed at the effective dose (1.47 g/kg) for antihyperglycemic property according to Organisation for Economic Co-operation and Development (OECD)-425. The results showed that LLP demonstrated anti-inflammatory activities. It also showed no clinical signs of toxic effects and mortality in rats throughout 90 d. Thus, LLP could be classified in GHS category 5 which are of relatively low acute toxicity and then the lethal dose, 50% (LD50) cut off at 5000 mg/kg body weight to infinity (∞). Administration of LLP to the experimental rats significantly increased (p 
ISSN:0918-6158
1347-5215