Efficacy of antibiotic combinations in an experimental sepsis model with Pseudomonas aeruginosa

Summary This study aimed to compare the efficacy of fosfomycin, colistin, tobramycin and their dual combinations in an experimental sepsis model. After sepsis was established with a Pseudomonas aeruginosa isolate (P1), antibiotic-administered rats were divided into six groups: Fosfomycin, tobramycin...

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Bibliographic Details
Published in:Brazilian journal of microbiology 2023-12, Vol.54 (4), p.2817-2826
Main Authors: Aktas, Zerrin, Sonmez, Nese, Oksuz, Lutfiye, Boral, Ozden, Issever, Halim, Oncul, Oral
Format: Article
Language:English
Subjects:
Animal tissues
Antibiotics
Bacteria
Biofilms
Biomedical and Life Sciences
Clinical Microbiology - Research Paper
Colistin
Colonies
Effectiveness
Food Microbiology
Fosfomycin
In vivo methods and tests
Kidneys
Life Sciences
Liver
Lungs
Medical Microbiology
Microbial Ecology
Microbial Genetics and Genomics
Microbiology
Mycology
Pseudomonas aeruginosa
Samples
Sepsis
Spleen
Statistical analysis
Tobramycin
Translocation
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Summary:Summary This study aimed to compare the efficacy of fosfomycin, colistin, tobramycin and their dual combinations in an experimental sepsis model. After sepsis was established with a Pseudomonas aeruginosa isolate (P1), antibiotic-administered rats were divided into six groups: Fosfomycin, tobramycin, colistin and their dual combinations were administered by the intravenous or intraperitoneal route to the groups. The brain, heart, lung, liver, spleen and kidney tissues of rats were cultured to investigate bacterial translocation caused by P1. Given the antibiotics and their combinations, bacterial colony counts in liver tissues were decreased in colistin alone and colistin plus tobramycin groups compared with control group, but there were no significant differences. In addition, a non-statistical decrease was found in the spleen tissues of rats in the colistin plus tobramycin group. There was a > 2 log 10 CFU/ml decrease in the number of bacterial colonies in the kidney tissues of the rats in the fosfomycin group alone, but the decrease was not statistically significant. However, there was an increase in the number of bacterial colonies in the spleen and kidney samples in the group treated with colistin as monotherapy compared to the control group. The number of bacterial colonies in the spleen samples in fosfomycin plus tobramycin groups increased compared to the control group. Bacterial colony numbers in all tissue samples in the fosfomycin plus colistin group were found to be close to those in the control group. Colistin plus tobramycin combinations are effective against P. aeruginosa in experimental sepsis, and clinical success may be achieved. New in vivo studies demonstrating the ability of P. aeruginosa to biofilm formation in tissues other than the lung are warranted in future.
ISSN:1517-8382
1678-4405
DOI:10.1007/s42770-023-01141-9