Insights into the Biological Activity and Cytotoxic Mechanism of Epimedium pubigerum

In this work, the phytochemical characterization, biological activity, and cytotoxic mechanism of aerial and rhizome methanol extracts (SME and RME) of Epimedium pubigerum were investigated to demonstrate its potential usage in the treatment of lung cancer. LC‐HRMS analysis, total phenolic/flavonoid...

Full description

Saved in:
Bibliographic Details
Published in:Chemistry & biodiversity 2023-11, Vol.20 (11), p.e202301003-n/a
Main Authors: Seyhan, Gökçe, Akkaya, Didem, Kolci, Kübra, Reis, Rengin, Yazici, Nurdan, Nur Barut, Elif, Barut, Burak
Format: Article
Language:English
Subjects:
apoptosis
Aquatic plants
Assaying
Biological activity
Caspase-3
Cytochrome c
Cytotoxicity
Deoxyribonucleic acid
DNA
Epimedium
Epimedium pubigerum
Flavonoids
Fumaric acid
LC-HRMS
Lung cancer
p53
Phenolic compounds
Phenols
Rhizomes
Scavenging
Western blotting
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:In this work, the phytochemical characterization, biological activity, and cytotoxic mechanism of aerial and rhizome methanol extracts (SME and RME) of Epimedium pubigerum were investigated to demonstrate its potential usage in the treatment of lung cancer. LC‐HRMS analysis, total phenolic/flavonoid content assay, DPPH radical scavenging assay, DNA interaction, cytotoxicity, and western blotting were investigated using different methods. Fumaric acid was found to be the most abundant compound in both extracts. SME and RME were cytotoxic on A549 cells concentration‐dependently. Also, in vitro scratch assay showed that SME and RME led to a significant anti‐migratory effect at 1 mg/mL. Cytochrome c, p53, and caspase 3 expression significantly increased in the presence of RME compared to the control. All of these results claimed that RME might be suggested as a theoretically more effective phytotherapeutic agent for lung cancer compared to the effect seen with the SME.
ISSN:1612-1872
1612-1880
DOI:10.1002/cbdv.202301003