Sensitive and selective alternariol analysis by a newly developed monoclonal antibody-based immunoassay
Alternariol is a toxic secondary metabolite produced by Alternaria fungi. Nowadays, this mycotoxin can be found in many products of plant origin at concerning concentrations. The aim of the present study was to develop a highly sensitive and selective immunochemical method for the analysis of altern...
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| Published in: | Talanta (Oxford) 2024-01, Vol.267, p.125263-125263, Article 125263 |
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| Main Authors: | , , , , |
| Format: | Article |
| Language: | eng |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Alternariol is a toxic secondary metabolite produced by Alternaria fungi. Nowadays, this mycotoxin can be found in many products of plant origin at concerning concentrations. The aim of the present study was to develop a highly sensitive and selective immunochemical method for the analysis of alternariol. To this end, hapten synthesis was carried out from the scratch and specific high-affinity monoclonal antibodies to alternariol were generated by using, for the first time, alternariol bioconjugates with unambiguous linker tethering sites. A novel indirect enzyme-linked immunosorbent assay that incorporated a rationally designed heterologous conjugate was developed. The optimized assay showed an outstanding half-maximal inhibition concentration for alternariol below 0.04 ng/mL, and no cross-reactivity with alternariol monomethyl ether was observed. Very good recovery values and coefficients of variation were obtained from the analysis of alternariol-fortified fruit and cereal flour samples. Finally, alternariol was quantitatively determined in pears that had been previously infected with Alternaria alternata, showing excellent correlation with liquid chromatography results.
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•A novel hapten with the linker at C-1 was designed for the mycotoxin alternariol.•Specific, high-affinity monoclonal antibodies to alternariol were generated.•A heterologous immunoassay was optimized for alternariol analysis.•The LOQ was 1 ng/mL for oats and buckwheat flour and 10 ng/mL for pears.•The immunoassay was statistically validated using pears infected with Alternaria. |
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| ISSN: | 0039-9140 1873-3573 |