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Metabolic and genomic analysis deciphering biocontrol potential of endophytic Streptomyces albus RC2 against crop pathogenic fungi
Plant diseases caused by phytopathogenic fungi are one of the leading factors affecting crop loss. In the present study, sixty-one Streptomyces strains were screened for their antifungal activity against relevant wide range fungal pathogens prominent in Vietnam, namely Lasiodiplodia theobromae , Fus...
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Published in: | Brazilian journal of microbiology 2023-12, Vol.54 (4), p.2617-2626 |
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Main Authors: | , , , , , , , , , |
Format: | Article |
Language: | English |
Subjects: | |
Citations: | Items that this one cites Items that cite this one |
Online Access: | Get full text |
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Summary: | Plant diseases caused by phytopathogenic fungi are one of the leading factors affecting crop loss. In the present study, sixty-one
Streptomyces
strains were screened for their antifungal activity against relevant wide range fungal pathogens prominent in Vietnam, namely
Lasiodiplodia theobromae
,
Fusarium fujikuroi
, and
Scopulariopsis gossypii
. Endophytic strain RC2 was the most effective strain in the mycelial inhibition of the tested fungi. Based on phenotypic characteristics, 16S rDNA gene analysis, and genomic analysis, strain RC2 belonged to
Streptomyces albus
. An ethyl acetate extract of
S. albus
RC2 led to the strong growth inhibition of
S. gossypii
Co1 and
F. fujikuroi
L3, but not
L. theobromae
N13. The crude extract also suppressed the spore germination of
S. gossypii
Co1 and
F. fujikuroi
L3 to 92.4 ± 3.2% and 87.4% ± 1.9%, respectively. In addition, the RC2 extract displayed potent and broad-spectrum antibacterial activity against
Escherichia coli
,
Pseudomonas aeruginosa
, and the phytopathogenic bacteria
Ralstonia solanacearum
and
Xanthomonas oryzae
. The genome of strain RC2 was sequenced and revealed the presence of 15 biosynthetic gene clusters (BGCs) with similarities ≥ 45% to reference BGCs available in the antiSMASH database. The UPLC-HRMS analysis led to the identification of 8 other secondary metabolites, which have not been reported in
S. albus
. The present study indicated that RC2 could be a potent biocontrol agent against phytopathogenic fungi. Further attention should be paid to antifungal metabolites without functional annotation, development of product prototypes, and greenhouse experiments to demonstrate effective control of the plant diseases. |
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ISSN: | 1517-8382 1678-4405 |
DOI: | 10.1007/s42770-023-01134-8 |