Expression patterns and antifungal function study of KaSPI in Mythimna separata

Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of , the cDNA sequence of with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analys...

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Bibliographic Details
Published in:Bulletin of entomological research 2023-12, Vol.113 (6), p.756-766
Main Authors: Chen, Ya-Ru, Yang, Hong-Jia, Cha, Jin-Myong, Zhang, Xin-Xin, Fan, Dong
Format: Article
Language:English
Subjects:
Animals
Antifungal Agents - pharmacology
Female
Larva
Moths - genetics
Serine Peptidase Inhibitors, Kazal Type - pharmacology
Trypsin
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Summary:Kazal-type serine protease inhibitors (KaSPI) play important roles in insect growth, development, digestion, metabolism and immune defence. In this study, based on the transcriptome of , the cDNA sequence of with Kazal domain was uploaded to GenBank (MN931651). Spatial and temporal expression analysis showed that was expressed at different developmental stages and different tissues, and it was induced by 20-hydroxyecdysone in third-instar larvae of . After 24 h infection by , the expression level of and the corresponding MsKaSPI content were significantly up-regulated, being 6.42-fold and 1.91-fold to the control group, respectively, while the activities of serine protease, trypsin and chymotrypsin were inhibited. After RNA interference interfered with for 6 h, the expression decreased by 73.44%, the corresponding content of MsKaSPI protein decreased by 55.66% after 12 h, and the activities of serine protease and trypsin were significantly enhanced. Meanwhile, both the larval and pupal stages of were prolonged, the weights were reduced and the number of eggs per female decreased by 181. infection also increased the mortality of -silenced by 18.96%. These prove can not only result in slow growth and low fecundity of by regulating the activity of related protease, but also participate in the resistance to pathogenic fungi by regulating the serine protease inhibitor content and the activities of related serine protease.
ISSN:0007-4853
1475-2670
DOI:10.1017/S000748532300041X