Male vitellogenin regulates gametogenesis through a testis‐enriched big protein in Chrysopa pallens

In insects, vitellogenin (Vg) is generally viewed as a female‐specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg‐deficient male downre...

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Bibliographic Details
Published in:Insect molecular biology 2024-02, Vol.33 (1), p.17-28
Main Authors: Liu, Xiao‐ping, Liu, Chang‐yan, Feng, Yan‐jiao, Guo, Xing‐kai, Zhang, Li‐sheng, Wang, Meng‐qing, Li, Yu‐yan, Zeng, Fan‐rong, Nolan, Tony, Mao, Jian‐jun
Format: Article
Language:English
Subjects:
Animals
C. pallens
Chrysopa pallens
Down-regulation
Embryos
Female
Females
Gametogenesis
Insecta - genetics
Insects
Male
Males
oogenesis
Organelles
Proteins
Reproduction
RNA-mediated interference
Seminal fluid
Spermatogenesis
Testes
Testis
Transcriptomes
Vitellogenin
Vitellogenins - genetics
Vitellogenins - metabolism
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Summary:In insects, vitellogenin (Vg) is generally viewed as a female‐specific protein. Its primary function is to supply nutrition to developing embryos. Here, we reported Vg from the male adults of a natural predator, Chrysopa pallens. The male Vg was depleted by RNAi. Mating with Vg‐deficient male downregulated female Vg expression, suppressed ovarian development and decreased reproductive output. Whole‐organism transcriptome analysis after male Vg knockdown showed no differential expression of the known spermatogenesis‐related regulators and seminal fluid protein genes, but a sharp downregulation of an unknown gene, which encodes a testis‐enriched big protein (Vcsoo). Separate knockdown of male Vg and Vcsoo disturbed the assembly of spermatid cytoplasmic organelles in males and suppressed the expansion of ovary germarium in mated females. These results demonstrated that C. pallens male Vg signals through the downstream Vcsoo and regulates male and female reproduction. Characterization of a vitellogenin (Vg) in Chrysopa pallens males. New functions of insect Vg: regulation of spermatid cytoplasmic organelle assembly and ovarian stem cell differentiation. C. pallens male Vg functions through a testis‐enriched big protein.
ISSN:0962-1075
1365-2583
DOI:10.1111/imb.12873