Comparison of 16S rRNA gene sequencing microbiota among children with serological IgE-mediated food hypersensitivity

We hypothesized that specific food hypersensitivity (FH) in children is linked to specific gut microbiota. The aim of our study was to quantify and evaluate differences in gut microbial composition among children with different IgE-mediated FH. Children (n = 81) aged 18 to 36 months were enrolled, f...

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Published in:Pediatric research 2024-01, Vol.95 (1), p.241-250
Main Authors: Chen, Chien-Chang, Huang, Jing-Long, Chen, Kun-Jei, Kong, Man-Shan, Hua, Man-Chin, Yeh, Yuan-Ming, Chang, Hung-Ju
Format: Article
Language:English
Subjects:
Allergens
Feces
Firmicutes - genetics
Food
Food Hypersensitivity
Gastrointestinal Microbiome - genetics
Genes, rRNA
Humans
Immunoglobulin E
Microbiota
Pediatrics
RNA, Ribosomal, 16S - genetics
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Summary:We hypothesized that specific food hypersensitivity (FH) in children is linked to specific gut microbiota. The aim of our study was to quantify and evaluate differences in gut microbial composition among children with different IgE-mediated FH. Children (n = 81) aged 18 to 36 months were enrolled, fecal samples of 57 children with FH and 24 healthy children were evaluated using next-generation sequencing. Individual microbial diversity and composition were analyzed via targeting the 16 S rRNA gene hypervariable V3-V5 regions. Children with IgE-mediated FH (in milk, egg white, soy) had significantly lower gut microbiota diversity and richness than healthy children. Children with IgE-mediated FH exhibited relatively high abundances of Firmicutes and relative underrepresentation of the phylum Bacteroidetes. We observed significant increases in relative abundances of Ruminococcaceae, Clostridiaceae, and Erysipelotrichaceae (p 
ISSN:0031-3998
1530-0447
DOI:10.1038/s41390-023-02735-7