Use of silicon and GaAs pixel detectors for digital autoradiography

Solid state detectors made of Si (4.8/spl times/8 mm/sup 2/) and GaAs (6.4/spl times/8 mm/sup 2/) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelia...

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Bibliographic Details
Published in:IEEE transactions on nuclear science 1997-06, Vol.44 (3), p.929-933
Main Authors: Amendolia, S.R., Beccherle, R., Bertolucci, E., Bisogni, M.G., Bottigli, U., Campbell, M., Chesi, E., Ciocci, M.A., Conti, M., Da Via, C., Del Guerra, A., D'Auria, S., Fantacci, M.E., Gambaccini, M., Grossi, G., Heijen, E., Mancini, E., Marchesini, R., Middelkamp, P., O'Shea, V., Randaccio, P., Romeo, N., Rosso, V., Russo, P., Scharfetter, L., Smith, K., Snoeys, W., Stefanini, A.
Format: Article
Language:eng
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DNA
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Summary:Solid state detectors made of Si (4.8/spl times/8 mm/sup 2/) and GaAs (6.4/spl times/8 mm/sup 2/) pixel matrices bump-bonded to the Omega2 and Omega3 electronic read-out systems, developed at CERN for H.E.P. experiments, have been used to obtain autoradiographic images of clusters of human epithelial cells and DNA fragments separated via electrophoresis, both labeled with /sup 32/P. The system has shown a good minimum detectable activity per unit area of 2.10/sup -4/ cps mm/sup -2/, and has proved linear for a count rate in the range 0.2-20 cpa, typical of autoradiography. The pixel dimensions are 75/spl times/500 /spl mu/m/sup 2/ (Si-Omega2) and 50/spl times/500 /spl mu/m/sup 2/ (GaAs-Omega3), respectively. We are able to clearly localize clusters of cells which have incorporated the radioactive tracer and DNA fragments on an electrophoretic gel on paper (blots).
ISSN:0018-9499
1558-1578