Senescence of bone marrow mesenchymal stem cells in Wistar male rats receiving normal chow/high-calorie diets with/without vitamin D

Bone marrow mesenchymal stem cells (BM-MSCs) have a momentous function in the composition of the bone marrow microenvironment because of their many valuable properties and abilities, such as immunomodulation and hematopoiesis. The features and actions of MSCs are influenced by senescence, which may...

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Published in:Biogerontology (Dordrecht) 2023-10, Vol.24 (5), p.801-812
Main Authors: Agh, Fahimeh, Mousavi, Seyed Hadi, Aryaeian, Naheed, Amiri, Fatemehsadat, Jalilvand, Mohammad Reza, Hasani, Motahareh, Vahid, Farhad, Sepahvand, Fatemeh, Vosugh, Mehran
Format: Article
Language:English
Subjects:
Animals
Biomedical and Life Sciences
Bmi protein
BMI-1 gene
Bone composition
Bone marrow
Cell Biology
Cell cycle
Cyclin-Dependent Kinase Inhibitor p16
Developmental Biology
Diet
G1 phase
G2 phase
Gene expression
Geriatrics/Gerontology
Hemopoiesis
Immunomodulation
INK4a protein
Life Sciences
Male
MAP kinase
Mesenchymal Stem Cells
Microenvironments
Noncommunicable Diseases
p16 Protein
Rats
Rats, Wistar
Reactive Oxygen Species
Research Article
S phase
Senescence
Statistical analysis
Stem cells
Vitamin D
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Summary:Bone marrow mesenchymal stem cells (BM-MSCs) have a momentous function in the composition of the bone marrow microenvironment because of their many valuable properties and abilities, such as immunomodulation and hematopoiesis. The features and actions of MSCs are influenced by senescence, which may be affected by various factors such as nutritional/micronutrients status, e.g., vitamin D. This study aimed to examine the effects of a high-calorie diet (HCD) with/without vitamin D on BM-MSCs senescence. In the first phase, 48 middle-aged rats were fed a normal chow diet (NCD, n = 24) and an HCD (n = 24) for 26 weeks. Afterward, the rats in each group were randomly divided into three equal subgroups. Immediately, eight-rat from each diet group were sacrificed to assess the HCD effects on the first phase measurements. In the second phase, the remaining 4 groups of rats were fed either NCD or HCD with (6 IU/g) or without vitamin D (standard intake: 1 IU/g); in other words, in this phase, the animals were fed (a) NCD, (b) NCD plus vitamin D, (c) HCD, and (d) HCD plus vitamin D for 4 months. BM-MSCs were isolated and evaluated for P16 INK4a , P38 MAPK, and Bmi-1 gene expression, reactive oxygen species (ROS) levels, SA-β-gal activity, and cell cycle profile at the end of both phases. After 26 weeks (first phase), the ROS level, SA-β-gal-positive cells, and cells in the G1 phase were significantly higher in HCD-fed rats than in NCD-fed ones (P  0.05). Compared with the NCD-fed animals, P16 INK4a and P38 MAPK gene expression were up-regulated in the HCD-fed animals; also, Bmi-1 gene expression was down-regulated (P 
ISSN:1389-5729
1573-6768
DOI:10.1007/s10522-023-10048-9