A retrospective observational study to estimate the risk of HLA alloimmunization with blood transfusion: Can the risk be reduced by leucodepletion?

In this retrospective study, our aim was to find the effect of leucodepleted (LD) blood transfusions on the formation of anti-HLA-antibodies when compared to non-leucodepleted (non-LD) transfusions using Luminex-based method. In this study, Luminex single antigen bead assay (L-SAB) and HLA typing we...

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Published in:Immunobiology (1979) 2023-09, Vol.228 (5), p.152727-152727, Article 152727
Main Authors: Pandey, Prashant, Pande, Amit, Marik, Arghyadeep, Sinha, Vijay Kumar, Devra, Amit Kumar, Bhatt, Anil Prasad, Kumari, Supriya, Gajway, Swapnil Yashwant, Singh, Ravi Kumar, Mishra, Smriti, Jha, Shantanu
Format: Article
Language:English
Subjects:
Donor-specific antibodies
HLA
HLA-antibodies
Kidney transplantation
Mean fluorescence intensity
SAB assay
Solid-phase assay
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Summary:In this retrospective study, our aim was to find the effect of leucodepleted (LD) blood transfusions on the formation of anti-HLA-antibodies when compared to non-leucodepleted (non-LD) transfusions using Luminex-based method. In this study, Luminex single antigen bead assay (L-SAB) and HLA typing were performed on 310 patients. Test positivity rates (as MFI - Mean florescence intensity) were analyzed according to the different sensitization events and gender. Of the 310 patients included in the study, 58.06% (180) patients were male and 41.93% (130) were female. The average age of the patients was 42.86 (±12.37) years. In this study, test positivity rates were significantly lower in the patients who received LD RBC units than in those who received non-LD RBC units (28.43% = 29 of 102 Vs 55.22% = 74 of 134, p 
ISSN:0171-2985
1878-3279
DOI:10.1016/j.imbio.2023.152727