Screening and optimization of a water-soluble near-infrared fluorescent probe for drug-induced liver injury monitoring

Peroxynitrite (ONOO−) is a potential biomarker of drug-induced liver injury (DILI) and is involved in the process of DILI. Therefore, developing a reliable detection method for ONOO− will greatly contribute to ensuring drug safety and improving treatment efficiency. Here, based on the previous work,...

Full description

Saved in:
Bibliographic Details
Published in:Analytica chimica acta 2023-10, Vol.1276, p.341654-341654, Article 341654
Main Authors: Pan, Yanping, Yang, Qiuxing, Xu, Hong, Yuan, Zhenwei, Xu, Hui
Format: Article
Language:English
Subjects:
Animals
Chemical and Drug Induced Liver Injury - diagnostic imaging
Chemical and Drug Induced Liver Injury - etiology
Drug-induced liver injury
Fluorescent Dyes - toxicity
Fluorescent probe
Near-infrared
Optical Imaging
Peroxynitrite
Peroxynitrous Acid
Water-soluble
Zebrafish
Citations: Items that this one cites
Items that cite this one
Online Access:Get full text
Tags: Add Tag
No Tags, Be the first to tag this record!
Description
Summary:Peroxynitrite (ONOO−) is a potential biomarker of drug-induced liver injury (DILI) and is involved in the process of DILI. Therefore, developing a reliable detection method for ONOO− will greatly contribute to ensuring drug safety and improving treatment efficiency. Here, based on the previous work, two kinds of NIR fluorescence probes PN and SPN were developed with phenyl-hydrazine as the ONOO− recognition group, which based on two fluorophores RN and SRN that are stable to ONOO−. A sensitive NIR probe SPN with good water solubility, low detection limit and good biocompatibility was selected through in vitro spectral property screening. Further experimental results show that there is a good linear relationship between the response intensity of probe SPN to ONOO− and the concentration of ONOO−, and the detection limit can reach 19.7 nM. At the cellular level, probe SPN can achieve a good and specific response to endogenous and exogenous ONOO−. Also, the probe SPN can be used for imaging and detection of DILI in zebrafish level and small animal level, indicating that probe SPN can be used as a powerful tool for diagnosis of DILI and efficacy evaluation of therapeutic drugs. [Display omitted] •A NIR probe SPN with good water solubility, low limit of detection.•The probe SPN can detect DILI in zebrafish and mice.
ISSN:0003-2670
1873-4324
DOI:10.1016/j.aca.2023.341654