Photodynamic Inactivation of Mycobacterium tuberculosis Using Alluminium Phthalocyanine

In a series of in vitro experiments, the optimum regimes of laser treatment were determined for effective photodynamic inactivation of Mycobacterium tuberculosis at a constant dose of aluminum phthalocyanine. Reference laboratory drug-susceptible strain H37Rv and clinical isolates of M. tuberculosis...

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Bibliographic Details
Published in:Bulletin of experimental biology and medicine 2023-07, Vol.175 (3), p.367-370
Main Authors: Nikonov, S. D., Bredikhin, D. A., Belogorodtsev, S. N., Schwartz, Ya.Sh
Format: Article
Language:English
Subjects:
Aluminum
Antibiotics
Biomedical and Life Sciences
Biomedicine
Cancer
Cell Biology
Clinical isolates
Drug dosages
Drug resistance in microorganisms
Internal Medicine
Laboratory Medicine
Lasers
Methylene blue
Mycobacterium tuberculosis
Pathology
Photochemotherapy
Phthalocyanins
Radiation
Tuberculosis
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Summary:In a series of in vitro experiments, the optimum regimes of laser treatment were determined for effective photodynamic inactivation of Mycobacterium tuberculosis at a constant dose of aluminum phthalocyanine. Reference laboratory drug-susceptible strain H37Rv and clinical isolates of M. tuberculosis with varying degrees of resistance to antibiotics were used. Suspensions of M. tuberculosis were incubated with aluminum phthalocyanine in a concentration of 5 μg/ml and then subjected to photodynamic inactivation with high- or low- intensity laser irradiation at λ=662 nm at various parameters of light power density. Mycobacteria survival rate was assessed by CFU assay on solid media. It was shown that at the specified dose of the photosensitizer, the photodynamic inactivation of mycobacterium was characterized by inhibition and complete cessation of their growth depending on the dose density of the laser energy. Effective photodynamic inactivation started from a light dose density of 46.9 J/cm 2 at a radiation power of 0.01 W and from 56.25 J/cm 2 at a radiation power of 0.1 W. Photodynamic inactivation at low laser power is more effective against drug-susceptible strains of M. tuberculosis .
ISSN:0007-4888
1573-8221
DOI:10.1007/s10517-023-05869-0